| Autor: |
Larry I. Lipshultz, Jacki Abrams, Edward D. Kim, William W. Lin |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
The Journal of Urology. :1334-1336 |
| ISSN: |
0022-5347 |
| Popis: |
Purposes: We have recently demonstrated that computer assisted image analysis of paraffin embedded testicular tissue based on deoxyribonucleic acid content and morphology characteristics is an effective method for the quantitative assessment of spermatogenesis. We assess the use of testicular touch preparation image analysis as a technique for quantification of spermatogenesis.Materials and Methods: Air dried, touch imprints of testicular tissue from obstructed azoospermic and severely oligozoospermic patients were obtained at the time of biopsy. Image analysis using a filter based on deoxyribonucleic acid content and cellular morphological characteristics was performed on Feulgen stained touch preparation imprints as well as paraffin embedded sections.Results: Image analysis of 52 testicular touch preparations from 48 azoospermic or severely oligozoospermic men revealed significant differences (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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