Sequence Analysis of the Mouse RAG Locus lntergenic Region
| Autor: | Gillian E. Wu, D. A. Martin, S. L. Olsona, F. E. Bertrand Iii |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
Transcription Genetic Trout Sequence analysis Genes RAG-1 Molecular Sequence Data Restriction Mapping Immunology Locus (genetics) Biology Mice Intergenic region Transcription (biology) Animals 3' Untranslated Regions Gene Zebrafish Regulation of gene expression Genetics promoter Base Sequence RAG-1 Three prime untranslated region RAG-2 hemic and immune systems regulation Sequence Analysis DNA lymphopoiesis DNA-Binding Proteins Open reading frame Gene Expression Regulation enhancer lcsh:RC581-607 Transcription Factors Research Article Developmental Biology |
| Zdroj: | Clinical and Developmental Immunology, Vol 5, Iss 3, Pp 215-222 (1998) Developmental Immunology |
| ISSN: | 1740-2530 1740-2522 |
| Popis: | The recombination activating genes RAG-1 and RAG-2 are highly conserved throughout evolution and are necessary and essential for the DNA rearrangement of antigen-receptor gene segments. These convergently transcribed genes are expressed primarily by developing B and T lineage cells. In addition, recent data suggest that the RAG locus can be reactivated in mouse germinal center B cells. Despite these well-defined patterns of expression, little is known about mechanism(s) regulating transcription of the RAG locus. Experiments with a mouse fibroblast line stably transfected with a genomic fragment of the RAG locus suggest that the intergenic region between RAG-1 and RAG-2 may contain information modulating RAG transcription. In order to begin testing this hypothesis, we have sequenced the 7.0-kb RAG intergenic region of the mouse. The sequence did not contain open reading frames larger than 60 amino acids. Analysis with GCG software identified several potential transcription-factor binding sequences within this region. Many of these are associated with transcriptional regulation of the Ig locus. |
| Databáze: | OpenAIRE |
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