Clofibrate elevates enzyme activities of the tricarboxylic acid cycle in rat liver
| Autor: | M Nikfardjam, D Schmid, Karl Kremser, Christiane Prager, Robert Kramar, M Untersalmberger, H J Schön |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1993 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Clofibrate biology Dehydrogenase Cell Biology QD415-436 Malate dehydrogenase Biochemistry Citric acid cycle Endocrinology Isocitrate dehydrogenase Enzyme chemistry Internal medicine Fumarase medicine biology.protein Citrate synthase medicine.drug |
| Zdroj: | Journal of Lipid Research, Vol 34, Iss 3, Pp 359-364 (1993) |
| ISSN: | 0022-2275 |
| Popis: | Activities of the tricarboxylic acid cycle enzymes were measured in subcellular fractions of liver from rats that had been fed clofibrate for 3 weeks. Large changes in these activities per gram tissue were found in the large particle fraction, which also showed an increase in total protein concentration of 76% under clofibrate treatment. The three regulatory enzymes of the cycle, namely citrate synthase, NAD+-linked isocitrate dehydro- genase, and 2-oxoglutarate dehydrogenase were significantly en- hanced by 24% (P < 0.02), 54% (P < 0.02), and 153% (P < 0.005), respectively. Fumarase and malate dehydrogenase rose by 71% (P < 0.005) and 95% (P < 0.02), whereas suc- cinate dehydrogenase remained unchanged. I Enhancement of the citrate synthase, NAD-isocitrate dehydrogenase, and 2-oxoglutarate dehydrogenase may play a role in decreasing in- tracellular availability of acetyl-coA for lipid metabolism.- Prager, C., H. J. Schon, M. Nikfardjam, D. Schmid, M. Untersalmberger, K. Kremser, and R. Kramar. Clofibrate ele- vates enzyme activities of the tricarboxylic acid cycle in rat liver. J. Lipid Res. 1993. 34: 359-364. Supplementary key wods clofibrate feeding enzyme induction citrate synthase NAD+-linked isocitrate dehydrogenase 2-oxoglu- tarate dehydrogenase |
| Databáze: | OpenAIRE |
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