Bacitracin attenuates haemolysis-induced insulin degradation during insulin sensitivity testing: Repurposing an old drug for use in metabolic research
| Autor: | Andrew P. Demidowich, Sheila M. Brady, Jordan A. Levine, Cheryl D. Johnson, Steven J. Soldin, Jack A. Yanovski |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adult
medicine.medical_specialty Endocrinology Diabetes and Metabolism medicine.medical_treatment 030209 endocrinology & metabolism Bacitracin 030204 cardiovascular system & hematology Hemolysis Article 03 medical and health sciences 0302 clinical medicine Endocrinology Insulin resistance Internal medicine Internal Medicine medicine Insulin-degrading enzyme Glucose homeostasis Humans Insulin business.industry Drug Repositioning Haemolysis medicine.disease In vitro stomatognathic diseases Pharmaceutical Preparations Insulin Resistance business Intracellular medicine.drug |
| Zdroj: | Diabetes Obes Metab |
| ISSN: | 1463-1326 |
| Popis: | AIMS: Hemolysis of serially-collected insulin serum samples frequently causes falsely-low measured concentrations due to release of intracellular insulin degrading enzyme (IDE). We investigated if bacitracin, an in vitro IDE inhibitor, could prevent hemolysis-induced insulin degradation during insulin sensitivity testing. MATERIALS AND METHODS: Blood samples were collected from adults undergoing serial sampling for insulin sensitivity. A dose-finding study measured insulin from experimentally-hemolyzed samples containing five bacitracin concentrations (0–2.5g/L) and from non-experimentally-hemolyzed samples. To confirm utility of bacitracin in the clinical setting, we compared insulin in samples collected with and without 1g/L bacitracin from a frequently sampled intravenous glucose tolerance test (FSIVGTT), where hemolysis often occurs accidentally. RESULTS: In the dose-finding study, bacitracin 0.25g/L, 1g/L, and 2.5g/L all maximally prevented insulin degradation in experimentally-hemolyzed samples. Among FSIVGTT unintentionally-hemolyzed samples, insulin concentrations from bacitracin-containing samples were significantly higher than from those without bacitracin (p |
| Databáze: | OpenAIRE |
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