Molecular analyses of two bacterial sampling methods in ligature‐induced periodontitis in rats
| Autor: | Vanderlei Salvador Bagnato, Clovis Grecco, Laura Marise de Freitas, Carla Raquel Fontana, Nikolaos S. Soukos, Constantinos Boussios |
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| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), MIT, 111 Dana Research Center |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Gingival and periodontal pocket medicine.medical_treatment Dentistry sampling technique Biology Bacterial composition Dental plaque Bacterial counts molecular diagnostics 03 medical and health sciences 0302 clinical medicine medicine Ligature General Dentistry Periodontitis business.industry Sampling (statistics) 030206 dentistry Original Articles medicine.disease biofilm periodontal pathogens MICROBIOLOGIA ORAL 030104 developmental biology Checkerboard Original Article business |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Clinical and Experimental Dental Research |
| Popis: | Made available in DSpace on 2018-12-11T16:51:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The prevalence profile of periodontal pathogens in dental plaque can vary as a function of the detection method; however, the sampling technique may also play a role in determining dental plaque microbial profiles. We sought to determine the bacterial composition comparing two sampling methods, one well stablished and a new one proposed here. In this study, a ligature-induced periodontitis model was used in 30 rats. Twenty-seven days later, ligatures were removed and microbiological samples were obtained directly from the ligatures as well as from the periodontal pockets using absorbent paper points. Microbial analysis was performed using DNA probes to a panel of 40 periodontal species in the checkerboard assay. The bacterial composition patterns were similar for both sampling methods. However, detection levels for all species were markedly higher for ligatures compared with paper points. Ligature samples provided more bacterial counts than paper points, suggesting that the technique for induction of periodontitis could also be applied for sampling in rats. Our findings may be helpful in designing studies of induced periodontal disease-associated microbiota. Universidade Estadual Paulista (Unesp) Faculdade de Ciências Farmacêuticas Instituto de Física-Grupo de Óptica–Universidade de Sao Paulo USP Laboratory for Information and Decision Systems Massachusetts Institute of Technology MIT Department of Physics 111 Dana Research Center Universidade Estadual Paulista (Unesp) Faculdade de Ciências Farmacêuticas FAPESP: 2016/05345-4 |
| Databáze: | OpenAIRE |
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