Altered expression of miR-146a in myasthenia gravis
| Autor: | Yu-Zhong Wang, Fa-Fa Tian, Ning Zhang, Wenbin Zhou, Huan Yang, Junmei Zhang, Jiayin Lu, Jing Li, Mei Yan |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Peripheral blood mononuclear cell Flow cytometry Mice Young Adult Internal medicine Myasthenia Gravis medicine Animals Humans Receptors Cholinergic CD40 Antigens Cells Cultured B cell CD86 B-Lymphocytes CD40 biology medicine.diagnostic_test General Neuroscience NF-kappa B Middle Aged medicine.disease Myasthenia gravis Mice Inbred C57BL Toll-Like Receptor 4 Blot MicroRNAs Endocrinology medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Case-Control Studies B7-1 Antigen biology.protein Female B7-2 Antigen CD80 |
| Zdroj: | Neuroscience Letters. 555:85-90 |
| ISSN: | 0304-3940 |
| Popis: | The purpose of this study was to analyze the expression of miR-146a in PBMCs obtained from patients with myasthenia gravis (MG) and healthy controls and to investigate the effect of the inhibition of miR-146a on the activation of AchR specific B cells obtained from mice. The expression of miR-146a levels in PBMCs obtained from patients with MG and healthy controls were determined by qRT-PCR. MiR-146a's complementary fragment, AntagomiR-146a, was synthesized as inhibitor, and the nonfunctional fragment, which has similar construction to AntagomiR-146a, was synthesized as negative control inhibitor. The expression of miR-146a, CD40, CD80 and CD86 on AchR specific B cells were analyzed by qRT-PCR and flow cytometry. Western blotting was used to detect the expression of TLR4, NF-κB and Bcl-2 .The expression of miRNA-146a in PBMCs obtained from patients with MG was significantly upregulated compared to healthy controls (P0.01). Transfection with miR-146a inhibitor dramatically decreased expression of miR-146a, CD40, CD80, TLR4 and NF-κB on AchR specific B cells compared to mock transfected cells. We conclude that abnormal expression/regulation of miR-146a may play an important role in the regulation of AchR specific B cells and contribute to the pathogenesis of MG. |
| Databáze: | OpenAIRE |
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