Synthesis and characterisation of N-gene targeted NIR-II fluorescent probe for selective localisation of SARS-CoV-2
| Autor: | Maha Alafeef, Dipendra Dahal, Ketan Dighe, Parikshit Moitra, Dipanjan Pan, Zach Sheffield |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Metal Nanoparticles 02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences Catalysis chemistry.chemical_compound Mice Quantum Dots Materials Chemistry Animals Humans Lead sulfide Lung Fluorescent Dyes Mice Inbred BALB C Spectroscopy Near-Infrared SARS-CoV-2 Metals and Alloys RNA COVID-19 General Chemistry Oligonucleotides Antisense 021001 nanoscience & nanotechnology Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry Models Animal Ceramics and Composites Click chemistry Biophysics Click Chemistry 0210 nano-technology Molecular probe Ex vivo |
| Zdroj: | Chemical communications (Cambridge, England). 57(51) |
| ISSN: | 1364-548X |
| Popis: | Tracking the viral progression of SARS-CoV-2 in COVID-19 infected body tissues is an emerging need of the current pandemic. Imaging at near infrared second biological window (NIR-II) offers striking benefits over the other technologies to explore deep-tissue information. Here we design, synthesise and characterise a molecular probe that selectively targets the N-gene of SARS-CoV-2. Highly specific antisense oligonucleotides (ASOs) were conjugated to lead sulfide quantum dots using a UV-triggered thiol-ene click chemistry for the recognition of viral RNA. Our ex vivo imaging studies demonstrated that the probe exhibits aggregation induced NIR-II emission only in presence of SARS-CoV-2 RNA which can be attributed to the efficient hybridisation of the ASOs with their target RNA strands. |
| Databáze: | OpenAIRE |
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