Cloning, characterization and cadmium inducibility of metallothionein in the testes of the mudskipper Boleophthalmus pectinirostris
| Autor: | You-Fa Wang, Ling-Li Long, Wan-Xi Yang, Jun-Quan Zhu, Zhang Sheng, Guo-Di Liu, Ying-Li Han |
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| Rok vydání: | 2015 |
| Předmět: |
Untranslated region
Male Spermiogenesis RNA Mitochondrial Health Toxicology and Mutagenesis Biology Testis Metallothionein Animals Cloning Molecular Spermatogenesis Cloning Base Sequence Public Health Environmental and Occupational Health Fishes General Medicine Pollution Molecular biology Immunohistochemistry Open reading frame Gene Expression Regulation Cytoplasm Models Animal RNA Water Pollutants Chemical Cysteine Cadmium |
| Zdroj: | Ecotoxicology and environmental safety. 119 |
| ISSN: | 1090-2414 |
| Popis: | Metallothioneins (MTs) are cysteine-rich, low molecular weight, and heavy metal-binding protein molecules. MT participates in metallic homeostasis and detoxification in living animals due to its abundant cysteine. In order to investigate the functions of MT during spermiogenesis in the mudskipper (Boleophthalmus pectinirostris), we identified the MT complete which contains: an 83bp 5' untranslated region, a 110bp 3' untranslated region, and a 183bp open reading frame. The protein alignment between MT sequences of other species shows a high similarity and a strong identity in cysteine residues vital for the metal-binding affinity of MT. The localizations of MT were mainly in the cytoplasm of germinal cells, indicating a role in spermatogenesis and testis protection. After the cadmium (Cd) exposure, the testis presents abnormal morphology and MT mRNA expression, both of which indicate a sensitive response of testis MT to Cd. Therefore, we suggest that MTs play an important role in spermatogenesis and testes protection against Cd toxicity in B. pectinirostris. |
| Databáze: | OpenAIRE |
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