Changes in the Muscarinic Receptors on the Colonic Smooth Muscles of Rats with Spinal Cord Injury

Autor:	Hyun Park, Yong Sung Kim, Jung Taek Oh, Eul sik Choi, Moon Young Lee, Min Cheol Joo
Rok vydání:	2011
Předmět:	Muscarinic receptor subtype Pathology medicine.medical_specialty Colon business.industry Rehabilitation Spinal cord injury M3 M2 medicine.disease digestive system diseases Muscarinic acetylcholine receptor medicine Original Article sense organs business Receptor Acetylcholine medicine.drug
Zdroj:	Annals of Rehabilitation Medicine
ISSN:	2234-0653 2234-0645
DOI:	10.5535/arm.2011.35.5.589
Popis:	Objective To investigate changes in (1) the colonic response to acetylcholine (Ach), (2) the muscarinic (M) receptors in the colon, and (3) the levels of colonic contraction-related proteins after a spinal cord injury (SCI). Method We divided 16 Sprague-Dawley rats into 2 groups: the control group and the SCI group. A spinal cord transection was performed surgically at the T10 vertebral level. After 1 week, the entire colon was divided into 2 segments, the proximal and distal colon. Each segment was mounted in a longitudinal or circular muscle direction in a 10-ml organ bath. We determined the intergroup differences as percentage changes in contractility after Ach treatment alone, Ach treatment with M2 receptor antagonist (AQ-RA741) pretreatment, and Ach treatment with M3 receptor antagonist (4-DAMP) pretreatment. Western blot analyses were performed to determine the expression level of RhoA, and heat shock protein 27 (HSP27). Results Compared to the control rats, the SCI rats showed an increased response to Ach along both the directions in the proximal colon (p
Databáze:	OpenAIRE
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