Sentinel lymph node analysis in breast cancer: contribution of one-step nucleic acid amplification (OSNA)

Autor: Habiba Mesbah, Gwenola Gandon, Cécile Bendavid Athias, Vincent Lavoué, Patrick Tas, Véronique Quillien, Jean Lévêque, Florence Godey, Philippe Porée
Přispěvatelé: Service de Biologie, CRLCC Eugène Marquis (CRLCC), Département de chirurgie, Service de Gynécologie et Obstétrique [Rennes] = Gynaecology [Rennes], CHU Pontchaillou [Rennes], De Villemeur, Hervé
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Adult
Cancer Research
medicine.medical_specialty
Pathology
Sentinel lymph node
Breast Neoplasms
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.MHEP.GEO]Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
Metastasis
Cohort Studies
03 medical and health sciences
0302 clinical medicine
Breast cancer
[SDV.CAN] Life Sciences [q-bio]/Cancer
Humans
Medicine
Neoplasm Invasiveness
Stage (cooking)
Macrometastasis
Aged
Neoplasm Staging
030304 developmental biology
0303 health sciences
Sentinel Lymph Node Biopsy
business.industry
Micrometastasis
Axillary Lymph Node Dissection
Nucleic acid amplification technique
Middle Aged
Prognosis
medicine.disease
3. Good health
[SDV.MHEP.GEO] Life Sciences [q-bio]/Human health and pathology/Gynecology and obstetrics
Oncology
Lymphatic Metastasis
030220 oncology & carcinogenesis
Female
Lymph Nodes
Radiology
business
Nucleic Acid Amplification Techniques
Zdroj: Breast Cancer Research and Treatment
Breast Cancer Research and Treatment, Springer Verlag, 2012, 131 (2), pp.509-16. ⟨10.1007/s10549-011-1808-4⟩
ISSN: 0167-6806
1573-7217
DOI: 10.1007/s10549-011-1808-4⟩
Popis: International audience; One-step nucleic acid amplification (OSNA, Sysmex, Kobe, Japan) offers an excellent opportunity for accurate exhaustive sentinel lymph node (SLN) examination in breast cancer patients. Calibrated with conventional postoperative histology, this molecular technique yields comparable results intraoperatively, expressed as micrometastasis, macrometastasis or no metastasis depending on the CK19 mRNA copy number amplified in SLN lysates. We applied OSNA to detect metastasis in 810 SLNs from 367 patients with early stage breast cancer. We compared the rate of OSNA-positive SLNs in patients with invasive breast cancer (< 2 cm) versus the rate observed in a historical cohort using conventional histological examination of SLNs. No significant difference was observed, the OSNA assay was positive in 24.4% of patients, compared with positive histology in 24.8% in the historical cohort if including patients with isolated tumour cell (ITC) and in 23.4% excluding them. Opportunities for optimised patient management using OSNA are discussed: intraoperative detection of OSNA-positive SLNs enables axillary lymph node dissection (ALND) during the same procedure; standard OSNA techniques enable the establishment of homogeneous groups based on examination of whole SLNs for valid comparisons between different centres.
Databáze: OpenAIRE
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