The Paf1 complex represses ARG1 transcription in Saccharomyces cerevisiae by promoting histone modifications
| Autor: | Elia M. Crisucci, Karen M. Arndt |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Histone H3 Lysine 4
Saccharomyces cerevisiae Proteins Amino Acid Transport Systems Transcription Genetic Saccharomyces cerevisiae Biology Microbiology complex mixtures Methylation Histones Gene Knockout Techniques Genes Reporter Gene Expression Regulation Fungal Histone methylation Histone H2A Histone H2B Histone code Promoter Regions Genetic Molecular Biology Genetics Arginase EZH2 Ubiquitination Nuclear Proteins General Medicine Articles TATA-Box Binding Protein Recombinant Proteins Histone methyltransferase Heterochromatin protein 1 Protein Processing Post-Translational |
| Zdroj: | Eukaryotic cell. 10(6) |
| ISSN: | 1535-9786 |
| Popis: | The conserved multifunctional Paf1 complex is important for the proper transcription of numerous genes, and yet the exact mechanisms by which it controls gene expression remain unclear. While previous studies indicate that the Paf1 complex is a positive regulator of transcription, the repression of many genes also requires the Paf1 complex. In this study we used ARG1 as a model gene to study transcriptional repression by the Paf1 complex in Saccharomyces cerevisiae . We found that several members of the Paf1 complex contribute to ARG1 repression and that the complex localizes to the ARG1 promoter and coding region in repressing conditions, which is consistent with a direct repressive function. Furthermore, Paf1 complex-dependent histone modifications are enriched at the ARG1 locus in repressing conditions, and histone H3 lysine 4 methylation contributes to ARG1 repression. Consistent with previous reports, histone H2B monoubiquitylation, the mark upstream of histone H3 lysine 4 methylation, is also important for ARG1 repression. To begin to identify the mechanistic basis for Paf1 complex-mediated repression of ARG1 , we focused on the Rtf1 subunit of the complex. Through an analysis of RTF1 mutations that abrogate known Rtf1 activities, we found that Rtf1 mediates ARG1 repression primarily by facilitating histone modifications. Other members of the Paf1 complex, such as Paf1, appear to repress ARG1 through additional mechanisms. Together, our results suggest that Rtf1-dependent histone H2B ubiquitylation and H3 K4 methylation repress ARG1 expression and that histone modifications normally associated with active transcription can occur at repressed loci and contribute to transcriptional repression. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|