Histological Evaluation of the Effects of Cryopreservation in Bovine Ovarian Tissue
| Autor: | Aroldo F. Camargos, Agnaldo L. Silva-Filho, Eduardo Alves Bambirra, Maria das Graças R. S. Camargos, Rívia Mara Lamaita, Fernando M. Reis |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Substance-Related Disorders Short Communication Ovary Biology Urinalysis Truth Disclosure Cryopreservation Fresh Tissue Biopsy Genetics medicine Humans Medical History Taking Genetics (clinical) Histological examination Retrospective Studies medicine.diagnostic_test Oocyte Donation Illicit Drugs Ovarian tissue Obstetrics and Gynecology Reproducibility of Results General Medicine medicine.anatomical_structure Reproductive Medicine Tissue bank Standard protocol Female Developmental Biology |
| Popis: | The aim of this study was to show the effects of freezing and thawing in bovine ovarian tissue by histological analysis. Ten cortical slices (2-4 mm in diameter) were obtained from each ovary by tru-cut biopsy and randomly divided into two groups: five fragments were immediately processed as a fresh tissue control group, while the remaining 5 fragments were slowly frozen using DMSO plus sucrose as cryoprotectors, then stored for two weeks and quickly thawed. Histological examination of all cryopreserved ovarian fragments showed no damage in the structure of the organ. Furthermore, there was no difference in the average number of primordial and primary follicles between the two groups of ovarian tissue. These data suggest that the bovine ovary can be used as a suitable model to test new freezing and thawing procedures in search for a standard protocol of human ovary cryopreservation. |
| Databáze: | OpenAIRE |
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