An Arabidopsis divergent pumilio protein, APUM24, is essential for embryogenesis and required for faithful pre-rRNA processing
| Autor: | Nazia Abbasi, Youn-Il Park, Thiruvenkadam Shanmugam, Ho Bang Kim, Douglas G. Muench, Yeonhee Choi, Sung Aeong Oh, Nam-il Park, Guen Tae Park, Sang-Bong Choi, Hyung-Sae Kim, Soon Ki Park |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell division RNA Stability Mutant Arabidopsis Plant Science 03 medical and health sciences Auxin Genetics RNA Precursors RRNA processing chemistry.chemical_classification Ovule biology Arabidopsis Proteins Embryogenesis Nuclear Proteins RNA-Binding Proteins Embryo Cell Biology Ribosomal RNA biology.organism_classification Cell biology 030104 developmental biology chemistry RNA Ribosomal Mutation Cell Division Cell Nucleolus |
| Zdroj: | The Plant journal : for cell and molecular biology. 92(6) |
| ISSN: | 1365-313X |
| Popis: | Summary Pumilio RNA-binding proteins are largely involved in mRNA degradation and translation repression. However, a few evolutionarily divergent Pumilios are also responsible for proper pre-rRNA processing in human and yeast. Here, we describe an essential Arabidopsis nucleolar Pumilio, APUM24, that is expressed in tissues undergoing rapid proliferation and cell division. A T-DNA insertion for APUM24 did not affect the male and female gametogenesis, but instead resulted in a negative female gametophytic effect on zygotic cell division immediately after fertilization. Additionally, the mutant embryos displayed defects in cell patterning from pro-embryo through globular stages. The mutant embryos were marked by altered auxin maxima, which were substantiated by the mislocalization of PIN1 and PIN7 transporters in the defective embryos. Homozygous apum24 callus accumulates rRNA processing intermediates, including uridylated and adenylated 5.8S and 25S rRNA precursors. An RNA–protein interaction assay showed that the histidine-tagged recombinant APUM24 binds RNAin vitro with no apparent specificity. Overall, our results demonstrated that APUM24 is required for rRNA processing and early embryogenesis in Arabidopsis. |
| Databáze: | OpenAIRE |
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