Interference of the most frequent haemoglobin variants on quantification of HbA1c: comparison between the LC-MS (IFCC reference method) and three routinely used methods
| Autor: | Emmanuelle Guillard, Stéphane Jaisson, M. Tonye-Libyh, Philippe Gillery, Nathalie Leroy, C. Desroches |
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| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Heterozygote Endocrinology Diabetes and Metabolism Hemoglobins Abnormal Field methods High-performance liquid chromatography Haemoglobin variants Mass Spectrometry Endocrinology Capillary electrophoresis Liquid chromatography–mass spectrometry Internal Medicine medicine Humans In patient False Positive Reactions Chromatography High Pressure Liquid Glycated Hemoglobin Chromatography business.industry Homozygote Chromatography liquid Electrophoresis Capillary General Medicine Separation method business Chromatography Liquid |
| Zdroj: | Diabetesmetabolism. 39(4) |
| ISSN: | 1878-1780 |
| Popis: | Aim Assaying HbA 1c in patients with haemoglobin variants has long been a technical challenge, despite methodological advances that have progressively limited the problem. The purpose of this study was to evaluate the impact of the most frequent haemoglobin variants on three routine separation methods compared with the IFCC reference method. Patients Blood samples from heterozygous patients (AS, AC, AD, AE) were analyzed using the IFCC reference method (LC–MS), and the results compared with those obtained by capillary electrophoresis (CAPILLARYS 2 Flex Piercing, Sebia) and two HPLC methods using cation-exchange (Variant II, Bio-Rad) and affinity chromatography (Ultra 2 , Primus). Results HbA 1c values obtained by the IFCC reference method were comparable to those obtained by the three tested methods whatever the haemoglobin variant. Mean relative biases did not exceed the threshold of 7% (above which differences are generally considered clinically significant), although some individual values were above this limit with Variant II in samples with HbS and for all three methods in samples with HbE. Conclusion This comparative study of the LC–MS reference method and three field methods has demonstrated that these assays are not clinically influenced by the presence of the most common haemoglobin variants. The present results also confirm that the interpretation of HbA 1c values in patients with Hb variants remains complex and depends on the assays used and should, in some cases, take into account parameters other than analytical ones (such as differences in glycation rates and half-lives of haemoglobin variants). |
| Databáze: | OpenAIRE |
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