Electrotransformation of Xanthobacter autotrophicus GJ10 and other Xanthobacter strains
| Autor: | J. Swaving, Albert J.J. van Ooyen, Jan A.M. de Bont, Wilco van Leest |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Microbiology (medical)
Xanthobacter autotrophicus Electroporation Genetic transfer Xanthobacter autotrophicus GJ10 Cell concentration Biology biology.organism_classification Microbiology Pulse time pLAFR5 Transformation Transformation (genetics) Industrial Microbiology Biochemistry Industriële microbiologie Xanthobacter Molecular Biology Azotobacteraceae |
| Zdroj: | Journal of Microbiological Methods 25 (1996) Journal of Microbiological Methods, 25, 343-348 |
| ISSN: | 0167-7012 |
| Popis: | This paper describes the development of an electroporation procedure for Xanthobacter autotrophicus GJ10. Electroporation has not previously been described for this species. The procedure was optimized with respect to field strength, pulse time and DNA and cell concentration. High transformation efficiencies of up to 2×10 6 cells transformed per μg DNA were found. The conditions optimal for the X. autotrophicus GJ10 were used to electrotransform 9 other Xanthobacter strains 4 of which were transformed with high efficiencies. The electrotransformation procedure described here can be of great help for the genetic availability of Xanthobacter species. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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