Antimicrobial potential of resin matrices loaded with coffee compounds
Autor: | Laiza Tatiana Poskus, Jack L. Ferracane, Sarah Aquino de Almeida, Eduardo Moreira da Silva, Amanda Mahammad Mushashe, Rayane V de Almeida, Anderson A. Rocha, Jaime Dutra Noronha-Filho, Justin Merritt |
---|---|
Rok vydání: | 2020 |
Předmět: |
Materials science
Bisphenol Biomedical Engineering High-performance liquid chromatography Coffee Composite Resins Niacin Article Biomaterials Streptococcus mutans 03 medical and health sciences chemistry.chemical_compound Minimum inhibitory concentration 0302 clinical medicine Alkaloids Chlorogenic acid Anti-Infective Agents Trigonelline 030304 developmental biology 0303 health sciences Chromatography biology 030206 dentistry biology.organism_classification Antimicrobial chemistry Formazan Chlorogenic Acid |
Zdroj: | J Biomed Mater Res B Appl Biomater |
ISSN: | 1552-4981 |
Popis: | This study evaluated the biological behavior of the coffee compounds Trigonelline (T), chlorogenic acid (C), and nicotinic acid (N), correlating with their release from a resin matrix. Minimum inhibitory concentration (MIC) was evaluated against Streptococcus mutans UA159, and cytotoxicity was assessed by methyl tetrazolium salt on OD-21 cells. Resin matrices (bisphenol A-glycidyl-dimethacrylate/triethylene glycol-dimethacrylate 70/30 wt%, camphorquinone/ethyl 4-dimethyl aminobenzoate 0.5/1 wt%) were doped with coffee compounds in different concentrations (10/20/30/40/50 wt%), performing 15 groups (T10-T50, C10-C50, N10-N50), and a control group with no coffee compound. Degree of conversion (DC%) was analyzed by Fourier transform infrared spectroscopy. Antimicrobial properties were evaluated by bioluminescence (Luciferase assay). The release from loaded matrices was analyzed over time (24 hr, 6, 14, 21 and 28 days), using high-performance liquid chromatography (HPLC). Data were submitted to ANOVA/Tukey's test (α = 0.05). MIC for T and C was 6 mg/ml, and 4 mg/ml for N. None of them were cytotoxic. Only T50 and C50 showed lower DC% than control (α < 0.05). Some groups (T30/T40/T50/C40/C50/N50) were strongly antimicrobial, reducing bacterial activity approximately five times compared to control (α < 0.05). For T30, T40, T50, C40, and C50, the HPLC showed a release above or closer to MIC values mainly in 24 hr, but for N50, up to 28 days. In conclusion, the coffee compounds presented antimicrobial activity, depending on their concentration when added in resin matrices, being found a correlation with their release. |
Databáze: | OpenAIRE |
Externí odkaz: |