Quantitation of Acetyl Hexapeptide-8 in Cosmetics by Hydrophilic Interaction Liquid Chromatography Coupled to Photo Diode Array Detection
| Autor: | E. Tsirivas, Irene Panderi, Eleni Kalogria, Athanasia Varvaresou, Vasiliki Raikou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
media_common.quotation_subject
QC1-999 hydrophilic interaction liquid chromatography Filtration and Separation acetyl hexapeptide-3 Cosmetics Analytical Chemistry law.invention Argireline chemistry.chemical_compound law Ammonium formate QD1-999 media_common Chromatography Acetyl hexapeptide-3 Hydrophilic interaction chromatography Physics Cosmetic cream Photodiode Chemistry chemistry acetyl hexapeptide-8 chromatography oligopeptides cosmetics |
| Zdroj: | Separations; Volume 8; Issue 8; Pages: 125 Separations, Vol 8, Iss 125, p 125 (2021) |
| ISSN: | 2297-8739 |
| DOI: | 10.3390/separations8080125 |
| Popis: | Bioactive peptides are gaining more and more popularity in the research and development of cosmetic products with anti-aging effect. Acetyl hexapeptide-8 is a hydrophilic peptide incorporated in cosmetics to reduce the under-eye wrinkles and the forehead furrows. Hydrophilic interaction liquid chromatography (HILIC) is the separation technique of choice for analyzing peptides. In this work, a rapid HILIC method coupled to photodiode array detection operated at 214 nm was developed, validated and used to determine acetyl-hexapeptide-8 in cosmetics. Chromatography was performed on a Xbridge® HILIC BEH analytical column using as mobile phase a 40 mM ammonium formate water solution (pH 6.5)-acetonitrile mixture 30:70, v/v at flow rate 0.25 mL min−1. The assay was linear over the concentration range 20 to 30 μg mL−1 for the cosmetic formulations and 0.004 to 0.007% (w/w) for the cosmetic cream. The limits of quantitation for acetyl hexapeptide-8 were 1.5 μg mL−1 and 0.002% (w/w) for the assay of cosmetic formulations and cosmetic creams, respectively. The method was applied to the analysis of cosmetic formulations and anti-wrinkle cosmetic creams. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|