Mitochondrial, Acidic, and Cytosolic pHs Determination by 31P NMR Spectroscopy: Design of New Sensitive Targeted pH Probes
| Autor: | Sylvia Pietri, Ariane Atteia, Sophie Thétiot-Laurent, Marcel Culcasi |
|---|---|
| Přispěvatelé: | Institut de Chimie Radicalaire (ICR), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Weissig, Volkmar, Edeas, Marvin, ANR–09-BLAN-005-03,ROS SIGNAL,ROS SIGNAL ANR–09-BLAN-005-03 |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Subcellular pH
biology Acidic vacuoles Chemistry Mitochondria-targeted aminophosphonates Chlamydomonas reinhardtii [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology Nuclear magnetic resonance spectroscopy Mitochondrion biology.organism_classification Mitochondria Cytosolic pH Cytosol Inorganic phosphate Biochemistry Biophysics 31P NMR pH Probes |
| Zdroj: | Mitochondrial Medicine Weissig, Volkmar; Edeas, Marvin. Mitochondrial Medicine, II, Humana Press, pp.135-147, 2015, Manipulating Mitochondrial Function, ISBN 978-1-4939-2287-1. ⟨10.1007/978-1-4939-2288-8_11⟩ Methods in Molecular Biology ISBN: 9781493922871 |
| DOI: | 10.1007/978-1-4939-2288-8_11⟩ |
| Popis: | International audience; 31P nuclear magnetic resonance (NMR) is a unique technique to monitor noninvasively the energetics ofliving systems at real time through the detection of a variety of phosphorylated metabolites. Usingadequately designed α-aminophosphonates as external probes, we have shown earlier that 31P NMR canalso give access simultaneously to the accurate pH of cytosolic and acidic compartments in normal andstressed cultured cells or isolated perfused organs, a feature that was not possible using endogenous inorganicphosphate as the probe. More recently, we obtained a series of derivatives of these new pH probesthat incorporate a triphenylphosphonium cation as a specifi c vector to the mitochondrion. Here, wedescribe the synthesis, 31P NMR pH titrating properties in buffers, and application in cultures of the greenalga Chlamydomonas reinhardtii of two of these mitochondria-targeted pH probes in comparison with onenonvectorized, yet still informative α-aminophosphonate. |
| Databáze: | OpenAIRE |
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