Effects of heparin on inositol 1,4,5-trisphosphate and guanosine 5'-O-(3-thio triphosphate) induced calcium release in cultured smooth muscle cells from rabbit trachea
| Autor: | Jeremy P.T. Ward, Charles H. C. Twort, Lorna C. Chopra, Ian R. Cameron |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
GTP'
Inositol Phosphates Biophysics Guanosine chemistry.chemical_element Inositol 1 4 5-Trisphosphate Calcium In Vitro Techniques Biochemistry Calcium in biology chemistry.chemical_compound Structure-Activity Relationship medicine Animals Inositol Inositol phosphate Molecular Biology chemistry.chemical_classification Lagomorpha biology Dose-Response Relationship Drug Heparin Muscle Smooth Cell Biology Thionucleotides biology.organism_classification Molecular Weight Trachea chemistry Guanosine 5'-O-(3-Thiotriphosphate) Sugar Phosphates Guanosine Triphosphate Rabbits medicine.drug |
| Zdroj: | Biochemical and biophysical research communications. 163(1) |
| ISSN: | 0006-291X |
| Popis: | The effects of heparin on intracellular calcium release in monolayers of permeabilised cultured rabbit smooth muscle cells were determined using 45 Ca effluxes. Low molecular weight heparin inhibited inositol 1,4,5- trisphosphate (InsP 3 ) induced Ca 2+ release (IC 50 = 0.8μg/ml), but not guanosine 5′-O-(3-thio triphosphate) (GTPγS) stimulated Ca 2+ release. Only a small inhibition was noted with high molecular weight heparin and de-N-sulphated heparin, although chondroitin sulphate A potently inhibited the InsP 3 response. These results indicate the competitive and specific nature of the heparin effect and give information about the structure of the InsP 3 binding site. |
| Databáze: | OpenAIRE |
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