Thrombomodulin favors leukocyte microvesicle fibrinolytic activity, reduces NETosis and prevents septic shock-induced coagulopathy in rats
| Autor: | Mélanie Burban, Pierrick Le Borgne, Raphaël Clere-Jehl, Lelia Grunebaum, Fatiha Zobairi, Delphine Borgel, Ferhat Meziani, Julie Helms, Elsa P. Bianchini, Jean-Luc Diehl, Florence Toti |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment 030204 cardiovascular system & hematology Lung injury Critical Care and Intensive Care Medicine Thrombomodulin DIC 03 medical and health sciences 0302 clinical medicine Septic shock Fibrinolysis medicine Coagulopathy Disseminated intravascular coagulation business.industry Research Microvesicle lcsh:Medical emergencies. Critical care. Intensive care. First aid NETosis lcsh:RC86-88.9 medicine.disease 030104 developmental biology Hemostasis Immunology Immunothrombosis business Microvesicles |
| Zdroj: | Annals of Intensive Care Annals of Intensive Care, Vol 7, Iss 1, Pp 1-10 (2017) |
| ISSN: | 2110-5820 |
| Popis: | Background Septic shock-induced disseminated intravascular coagulation is responsible for increased occurrence of multiple organ dysfunction and mortality. Immunothrombosis-induced coagulopathy may contribute to hypercoagulability. We aimed at determining whether recombinant human thrombomodulin (rhTM) could control exaggerated immunothrombosis by studying procoagulant responses, fibrinolysis activity borne by microvesicles (MVs) and NETosis in septic shock. Methods In a septic shock model after a cecal ligation and puncture-induced peritonitis (H0), rats were treated with rhTM or a placebo at H18, resuscitated and monitored during 4 h. At H22, blood was sampled to perform coagulation tests, to characterize MVs and to detect neutrophils extracellular traps (NETs). Lungs were stained with hematoxylin–eosin for inflammatory injury assessment. Results Coagulopathy was attenuated in rhTM-treated septic rats compared to placebo-treated rats, as attested by a significant decrease in procoagulant annexin A5+-MVs and plasma procoagulant activity of phospholipids and by a significant increase in antithrombin levels (84 ± 8 vs. 64 ± 6%, p |
| Databáze: | OpenAIRE |
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