| Autor: |
Massimo Guardigli, Patrizia Pasini, Elisa Michelini, Monica Musiani, Mara Mirasoli, Aldo Roda |
| Přispěvatelé: |
Roda A., Guardigli M., Pasini P., Mirasoli M., Michelini E., Musiani M. |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Analytica Chimica Acta. 541:25-35 |
| ISSN: |
0003-2670 |
| DOI: |
10.1016/j.aca.2004.11.083 |
| Popis: |
Bio- and chemiluminescence imaging techniques combine the high sensitivity of bio- and chemiluminescence detection with the ability of current light imaging devices to localize and quantify light emission down to the single-photon level. These techniques have been successfully exploited for the development of sensitive analytical methods relying on the evaluation of the spatial distribution of the light emitted from a target sample. In this paper, we report on recent applications of bio- and chemiluminescence imaging for in vitro and in vivo assays, including: quantitative assays performed in various analytical formats, such as microtiter plates, microarrays and miniaturized analytical devices, used in the pharmaceutical, clinical, diagnostic and environmental fields; luminescence imaging microscopy based on enzymatic, immunohistochemical and in situ hybridization reactions for the localization of metabolites, enzymes, antigens and gene sequences in cells and tissues; whole-body luminescence imaging in live animals for evaluating biological and pathological processes and for pharmacological studies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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