Polyethylenimine-mediated gene transfer into pancreatic tumor dissemination in the murine peritoneal cavity

Autor: Hatanaka K, Maeda M, Aoki K, Behr Jp, Remy Js, Teruhiko Yoshida, Furuhata S, Terada M
Přispěvatelé: Conception et application de molécules bioactives (CAMB), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2000
Předmět:
Genetic Markers
Pathology
medicine.medical_specialty
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
medicine.medical_treatment
Transgene
Genetic enhancement
Intraperitoneal injection
Genetic Vectors
Mice
Nude
[CHIM.THER]Chemical Sciences/Medicinal Chemistry
Biology
Polymerase Chain Reaction
03 medical and health sciences
Peritoneal cavity
Mice
0302 clinical medicine
Genetics
medicine
Animals
Humans
Polyethyleneimine
Luciferases
Molecular Biology
ComputingMilieux_MISCELLANEOUS
Peritoneal Neoplasms
030304 developmental biology
0303 health sciences
Mice
Inbred BALB C
Genetic transfer
Gene Transfer Techniques
DNA
Genetic Therapy
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
beta-Galactosidase
3. Good health
Pancreatic Neoplasms
medicine.anatomical_structure
Blood chemistry
030220 oncology & carcinogenesis
Cancer cell
Liposomes
Cancer research
Molecular Medicine
Pancreas
Zdroj: Gene Therapy
Gene Therapy, Nature Publishing Group, 2001, 8 (7), pp.508-514. ⟨10.1038/sj.gt.3301435⟩
ResearcherID
ISSN: 0969-7128
1476-5462
Popis: Although peritoneal dissemination of cancer cells often occurs at the advanced stages of pancreatic, gastric or ovarian cancers, no effective therapy has been established. Cationic lipid-mediated gene transfer into peritoneal dissemination may offer a prospect of safe therapies, but vector improvements are needed with regard to the efficiency and specificity of the gene transfer. In this study, the intraperitoneal injection of plasmid DNA:polyethylenimine (PEI) complexes into mice was evaluated as a gene delivery system for the peritoneal disseminations. The luciferase and beta-galactosidase genes were used as marker genes. PEI was more efficient than the cationic lipids examined in this study in vivo, and the transgene was preferentially expressed in the tumors. Although PCR analysis showed that the injected DNA was delivered to various organs, the distributed DNA became undetectable by 6 months after the gene transfer. Blood chemistry and histological analysis showed no significant toxicity in the injected mice. This study demonstrated that the intraperitoneal injection of DNA:PEI is a promising delivery method to transduce a gene into disseminated cancer nodules in the peritoneal cavity.
Databáze: OpenAIRE
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