Functional analysis of endo-1,4-β-glucanases in response to Botrytis cinerea and Pseudomonas syringae reveals their involvement in plant-pathogen interactions
| Autor: | Ivan Finiti, María de la O. Leyva, Jaime López-Cruz, Alan B. Bennett, M. Grant, Begonya Vicedo, C. Angulo, Pilar García-Agustín, Carmen González-Bosch, B. Calderan Rodrigues |
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| Rok vydání: | 2012 |
| Předmět: |
Mutant
endo-glucanases Arabidopsis Gene Expression Pseudomonas syringae Plant Science Cyclopentanes tomato Genes Plant Marker gene chemistry.chemical_compound Botrytis cinerea Cellulase Solanum lycopersicum Plant Growth Regulators Cell Wall Gene Expression Regulation Plant Botany Arabidopsis thaliana Oxylipins Glucans Ecology Evolution Behavior and Systematics Disease Resistance Plant Diseases Plant Proteins biology Jasmonic acid Callose fungi food and beverages General Medicine biology.organism_classification defence response Cell biology chemistry Host-Pathogen Interactions cell wall Botrytis Signal Transduction |
| Zdroj: | Plant biology (Stuttgart, Germany). 15(5) |
| ISSN: | 1438-8677 |
| Popis: | Plant cell wall modification is a critical component in stress responses. Endo-1,4-β-glucanases (EGs) take part in cell wall editing processes, e.g. elongation, ripening and abscission. Here we studied the infection response of Solanum lycopersicum and Arabidopsis thaliana with impaired EGs. Transgenic TomCel1 and TomCel2 tomato antisense plants challenged with Pseudomonas syringae showed higher susceptibility, callose priming and increased jasmonic acid pathway marker gene expression. These two EGs could be resistance factors and may act as negative regulators of callose deposition, probably by interfering with the defence-signalling network. A study of a set of Arabidopsis EG T-DNA insertion mutants challenged with P. syringae and Botrytis cinerea revealed that the lack of other EGs interferes with infection phenotype, callose deposition, expression of signalling pathway marker genes and hormonal balance. We conclude that a lack of EGs could alter plant response to pathogens by modifying the properties of the cell wall and/or interfering with signalling pathways, contributing to generate the appropriate signalling outcomes. Analysis of microarray data demonstrates that EGs are differentially expressed upon many different plant-pathogen challenges, hormone treatments and many abiotic stresses. We found some Arabidopsis EG mutants with increased tolerance to osmotic and salt stress. Our results show that impairing EGs can alter plant-pathogen interactions and may contribute to appropriate signalling outcomes in many different biotic and abiotic plant stress responses. |
| Databáze: | OpenAIRE |
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