Semi-interpenetrating polymer network cryogels based on poly(ethylene glycol) diacrylate and collagen as potential off-the-shelf platforms for cancer cell research
| Autor: | Alessandro Sannino, Marta Madaghiele, Luca Salvatore, Nunzia Gallo, Ugo Masullo, Stephan J. Reshkin, Rosa Angela Cardone, Maria Mastrodonato, Maria Raffaella Greco, Tiziano Verri, Anna Cavallo |
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| Přispěvatelé: | Masullo, Ugo, Cavallo, Anna, Greco, Maria Raffaella, Reshkin, Stephan J, Mastrodonato, Maria, Gallo, Nunzia, Salvatore, Luca, Verri, Tiziano, Sannino, Alessandro, Cardone, Rosa Angela, Madaghiele, Marta |
| Rok vydání: | 2020 |
| Předmět: |
Materials science
Surface Properties Cell Biomedical Engineering Cell Culture Techniques 02 engineering and technology 3D organotypic culture cancer cell research cryogels polyethylene glycol diacrylate porosity Polyethylene Glycols Biomaterials 03 medical and health sciences chemistry.chemical_compound Structure-Activity Relationship Cancer stem cell medicine Humans Interpenetrating polymer network 030304 developmental biology Cell Proliferation Mechanical Phenomena 0303 health sciences Matrigel 021001 nanoscience & nanotechnology Drug Combinations medicine.anatomical_structure chemistry Cell culture Cancer cell Biophysics Neoplastic Stem Cells Proteoglycans Collagen Laminin 0210 nano-technology Ethylene glycol Porosity Type I collagen Cryogels Carcinoma Pancreatic Ductal |
| Zdroj: | Journal of biomedical materials research. Part B, Applied biomaterialsREFERENCES. 109(9) |
| ISSN: | 1552-4981 |
| Popis: | In the present work, we investigated the potential of novel semi-interpenetrating polymer network (semi-IPN) cryogels, obtained through ultraviolet exposure of aqueous mixtures of poly(ethylene glycol) diacrylate and type I collagen, as tunable off-the-shelf platforms for 3D cancer cell research. We synthesized semi-IPN cryogels with variable collagen amounts (0.1% and 1% w/v) and assessed the effect of collagen on key cryogel properties for cell culture, for example, porosity, degradation rate and mechanical stiffness. Then, we investigated the ability of the cryogels to sustain the long-term growth of two pancreatic ductal adenocarcinoma (PDAC) cell populations, the parenchymal Panc1 cells and their derived cancer stem cells. Results revealed that both cell lines efficiently infiltrated, attached and expanded in the cryogels over a period of 14 days. However, only when grown in the cryogels with the highest collagen concentration, both cell lines reproduced their characteristic growth pattern previously observed in collagen-enriched organotypic cultures, biomimetic of the highly fibrotic PDAC stroma. Cellular preembedding in Matrigel, that is, the classical approach to develop/grow organoids, interfered with an efficient intra-scaffold migration and growth. Although preliminary, these findings highlight the potential of the proposed cryogels as reproducible and tunable cancer cell research platforms. |
| Databáze: | OpenAIRE |
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