Divalent Cations Suppress 3′,5′-Adenosine Monophosphate Accumulation by Stimulating a Pertussis Toxin-Sensitive Guanine Nucleotide-Binding Protein in Cultured Bovine Parathyroid Cells*
| Autor: | Joey V. Barnett, Edward M. Brown, Chu J. Chen, Dale A. Congo |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Adenosine monophosphate
medicine.medical_specialty Cations Divalent Dopamine Dinoprost medicine.disease_cause Pertussis toxin Divalent Parathyroid Glands chemistry.chemical_compound Cytosol Endocrinology GTP-Binding Proteins 1-Methyl-3-isobutylxanthine Internal medicine Cyclic AMP Extracellular medicine Animals Magnesium Virulence Factors Bordetella Phosphodiesterase inhibitor Cells Cultured chemistry.chemical_classification Adenosine Diphosphate Ribose Toxin Ionomycin Parathyroid chief cell Pertussis Toxin chemistry 3' 5'-Cyclic-AMP Phosphodiesterases Barium Strontium ADP-ribosylation Adenylate Cyclase Toxin Calcium Cattle Extracellular Space Ethers |
| Zdroj: | Endocrinology. 124:233-239 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-124-1-233 |
| Popis: | We used pertussis toxin to study the mechanism(s) by which divalent cations lower cellular cAMP content in bovine parathyroid cells. In cultured parathyroid cells, high extracellular Ca2+ (5 mM) or Mg2+ (5-10 mM) lowers dopamine-stimulated cAMP content by 70-90%. Pertussis toxin (0.5 microgram/ml) totally blocks the inhibitory effects of Ca2+ and Mg2+ on cAMP content. Ba2+ and Sr2+ (5 mM) also lower cAMP content by 80-90%, and this effect is, likewise, blocked by pertussis toxin. Pretreatment with pertussis toxin had no effect on the release of cAMP into the extracellular fluid. The toxin also did not modify phosphodiesterase activity in sonicates of parathyroid cells (42.68 +/- 3.26 vs. 47.00 +/- 2.82 pmol cAMP hydrolyzed/10(6) cells.20 min in control and toxin-treated cells, respectively). Moreover, addition of the phosphodiesterase inhibitor isobutyl-methylxanthine did not modify the inhibition of dopamine-stimulated cAMP accumulation by 5 mM Ca2+ in control cells (85% vs. 86% inhibition, respectively, with and without isobutylmethylxanthine). Pertussis toxin-catalyzed ADP ribosylation in homogenates of control cells demonstrated the presence of two substrates with mol wt of 40K and 41K. Preexposure of cells to pertussis toxin overnight resulted in the complete loss of both substrates on subsequent ADP ribosylation with [32P]NAD. Pertussis toxin pretreatment did not enhance adenylate cyclase activity indirectly via reducing the extracellular Ca2+-induced rise in cytosolic Ca2+, since the cytosolic Ca2+ level at 5 mM Ca2+ was about 60% higher in pertussis toxin-treated than in control cells (531 +/- 85 vs. 326 +/- 35 nM; P less than 0.05). In addition, ionomycin had no significant effect on cellular cAMP levels in control cells despite increasing the cytosolic Ca2+ concentration to levels as high as 1700 nM at 10(-5) M. Thus, changes in cytosolic Ca2+ phosphodiesterase activity, or efflux of cAMP from the cell cannot explain the inhibition of cAMP accumulation by divalent cations or the reversal of this effect by pertussis toxin. Instead, the present data suggest that extracellular divalent cations modulate the formation of cellular cAMP in parathyroid cells by a process involving a pertussis toxin-sensitive guanine nucleotide regulatory protein, presumably inhibition of adenylate cyclase by Gi via a receptor-like mechanism. |
| Databáze: | OpenAIRE |
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