The oral nitrate and nitrite intake in The Netherlands: evaluation of the results obtained by HPIC analysis of duplicate 24-hour diet samples collected in 1994
| Autor: | R. C. Schothorst, H. A. M. G. Vaessen |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Adolescent Daily intake Health Toxicology and Mutagenesis Ion chromatography Food Contamination Toxicology chemistry.chemical_compound Nitrate Humans Sample preparation Nitrite Nitrogen Compounds Nitrites Aged Netherlands Chromatography Nitrates Human blood Public Health Environmental and Occupational Health General Chemistry Middle Aged Diet Pays bas chemistry Biochemistry Chemistry (miscellaneous) Body Burden Female Duplicate diet Food Analysis Food Science |
| Zdroj: | Food additives and contaminants. 16(5) |
| ISSN: | 0265-203X |
| Popis: | In spring and autumn of 1994 duplicates of 24-h diets were collected from 123 respondents. One of the goals of this study was to determine the amount of nitrite and nitrate in the duplicates of 24-h diets to establish the oral daily intake of these analytes. For this purpose an HPIC/UV method for the determination of nitrate and nitrite in duplicate diets was developed and validated. The sample preparation procedure was derived from the in-house method used for the determination of nitrate and nitrite in human blood plasma. The sample is diluted with water, deproteinized with Carrez reagent, followed by chromatographic clean-up on an SPE C18-column. Both the nitrate and the nitrite results are quantitative. The recovery for nitrite was on average 104% (n = 21, spiking levels: 0.84-95 mg/kg) and for nitrate on average 103% (N = 21, spiking levels: 1.8-404 mg/kg). Samples of duplicates of 24-h diets were analysed according to the method developed. The median intake of nitrite calculated from the samples collected in spring 1994 was 0.6 mg/person day (range0.1-6.1 mg/person/day). For the samples collected in autumn 1994 these figures were0.2 mg/person/day (range0.1-16 mg/person/day). The mean intake of nitrate was 73 mg/person/day (range 7-322 mg/person/day) in spring 1994 and 87 mg/person/day (range 1-310 mg/person/day) in autumn 1994. The overall mean intake of nitrate in 1994 was 80 mg/person/day. The daily intake for nitrate was higher than that found in the duplicate diet study carried out in 1984/1985, when an average daily intake of 52 mg/person was measured. The intake of nitrite was also higher than found in the duplicate diets collected in 1984/1985. The findings of the study are discussed in the context of the ADI for nitrate and nitrite as well as the outcome of other recent European intake studies. |
| Databáze: | OpenAIRE |
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