Hybrid Erythrocyte Liposomes: Functionalized Red Blood Cell Membranes for Molecule Encapsulation
| Autor: | Christian Wagner, Alexander Kihm, Quinn Pauli, Kevin Yang, Sebastian Himbert, Maikel C. Rheinstädter, Matthew J. Blacker, Adree Khondker, Janos Juhasz, Harald D. H. Stöver, Mitchell Johnson, Sheilan Sinjari |
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| Rok vydání: | 2019 |
| Předmět: |
Erythrocytes
Biomedical Engineering General Biochemistry Genetics and Molecular Biology Biomaterials 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Delivery Systems Dynamic light scattering Phosphatidylcholine Zeta potential Humans 030304 developmental biology Phosphatidylglycerol 0303 health sciences Liposome Erythrocyte Membrane Dextrans Fluoresceins Nanostructures Dextran Membrane chemistry Drug delivery Liposomes Biophysics Synthetic Biology 030217 neurology & neurosurgery |
| Zdroj: | Advanced biosystems. 4(3) |
| ISSN: | 2366-7478 |
| Popis: | The modification of erythrocyte membrane properties provides a new tool towards improved drug delivery and biomedical applications. The fabrication of hybrid erythrocyte liposomes is presented by doping red blood cell membranes with synthetic lipid molecules of different classes (PC, PS, PG) and different degrees of saturation (14:0, 16:0-18:1). The respective solubility limits are determined, and material properties of the hybrid liposomes are studied by a combination of X-ray diffraction, epi-fluorescent microscopy, dynamic light scattering (DLS), Zeta potential, UV-vis spectroscopy, and Molecular Dynamics (MD) simulations. Membrane thickness and lipid orientation can be tuned through the addition of phosphatidylcholine lipids. The hybrid membranes can be fluorescently labelled by incorporating Texas-red DHPE, and their charge modified by incorporating phosphatidylserine and phosphatidylglycerol. By using fluorescein labeled dextran as an example, it is demonstrated that small molecules can be encapsulated into these hybrid liposomes. |
| Databáze: | OpenAIRE |
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