An effective live attenuated vaccine against Aeromonas veronii infection in the loach (Misgurnus anguillicaudatus)
| Autor: | Guanyi Xu, Xiaofeng Shan, Jing-rui Chen, Meng-yao Chen, Ling-cong Kong, Yu-xuan Xu, Yi-ming Wang, Hong-xia Ma, Yuanhuan Kang, Hai-Peng Zhang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_treatment Virulence Spleen Misgurnus Aeromonas veronii Aquatic Science Vaccines Attenuated Microbiology Lethal Dose 50 03 medical and health sciences Fish Diseases medicine Environmental Chemistry Animals Skin Attenuated vaccine biology 04 agricultural and veterinary sciences General Medicine biology.organism_classification Mucus Antibodies Bacterial Cypriniformes 030104 developmental biology medicine.anatomical_structure Cytokine Immunoglobulin M Liver Bacterial Vaccines 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Cytokines Antibody Gram-Negative Bacterial Infections |
| Zdroj: | Fishshellfish immunology. 104 |
| ISSN: | 1095-9947 |
| Popis: | Aeromonas veronii is a major pathogenic bacterium in humans and animals. When it causes outbreaks, there are enormous economic losses to the aquaculture industry. An effective live attenuated vaccine strain, ΔhisJ, was obtained in our previous studies by gene knockout in Aeromonas veronii TH0426 using the suicide vector pRE112. Here, we evaluated whether the live attenuated vaccine ΔhisJ was suitable for prevention of Aeromonas veronii infection by injection and immersion in loaches. Compared with that of the TH0426 wild-type strain, the virulence of the live vaccine was significantly weakened. Vaccine safety assessment results also indicated that 1 × 107 CFU/mL live vaccine was safe and did not induce clinical symptoms or obvious pathological changes. Additionally, after challenging loaches with Aeromonas veronii TH0426, the relative percent survival of the IN3 injection group was 65.66%, and that of the IM group was 50.78%. Our data show that the live attenuated vaccine ΔhisJ can improve the immune protection rate of loaches. Furthermore, increased enzyme activity parameters (SOD, LZM, ACP, and AKP) in the skin mucus, increased enzyme activity parameters (SOD, LZM, ACP, AKP, and GPx) in the serum, increased specific IgM antibodies and cytokine IL-1β contents in the serum, and increased cytokine (IL-15, pIgR, IL-1β, and TNF-α) expression in the liver and spleen were observed. These data are the first to indicate that the live attenuated vaccine ΔhisJ is suitable for the development of a safe and effective vaccine against Aeromonas veronii infection in loach aquaculture. |
| Databáze: | OpenAIRE |
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