Transient JMJD2B-Mediated Reduction of H3K9me3 Levels Improves Reprogramming of Embryonic Stem Cells into Cloned Embryos
| Autor: | Lawrence W. Chamley, Björn Oback, F. C. Oback, Jisha Antony, Götz Laible |
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| Rok vydání: | 2013 |
| Předmět: |
Jumonji Domain-Containing Histone Demethylases
Nuclear Transfer Techniques Histone lysine methylation Cloning Organism Green Fluorescent Proteins Gene Expression Methylation Histones Mice Animals Transgenes Epigenetics Molecular Biology Cells Cultured Embryonic Stem Cells biology Articles Cell Biology Cellular Reprogramming Embryonic stem cell Molecular biology Anti-Bacterial Agents Histone Doxycycline Histone methyltransferase Oocytes biology.protein Demethylase Female Reprogramming |
| Zdroj: | Molecular and Cellular Biology. 33:974-983 |
| ISSN: | 1098-5549 |
| Popis: | Correct reprogramming of epigenetic marks in the donor nuclei is crucial for successful cloning by nuclear transfer. Specific epigenetic modifications, such as repressive histone lysine methylation marks, are known to be very stable and difficult to reprogram. The discovery of histone lysine demethylases has opened up opportunities to study the effects of removing repressive histone lysine methylation marks in donor cells prior to nuclear transfer. In this study, we generated mouse embryonic stem (ES) cells for the inducible expression of JMJD2B (also known as KDM4B), a demethylase that primarily removes the histone-3 lysine-9 trimethylation (H3K9me3) mark. Induction of jmjd2b in the ES cells decreased total levels of H3K9me3 by 63%. When these cells were used for nuclear transfer, H3K9me3 levels were normalized within minutes following fusion with an enucleated oocyte. This transient reduction of H3K9me3 levels improved in vitro development into cloned embryos by 30%. |
| Databáze: | OpenAIRE |
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