Comprehensive quantification of ceramide species in human stratum corneum[S]
| Autor: | Tsuyoshi Oba, Yoshinori Masukawa, Naoki Kondo, Rika Homma, Junko Ishikawa, Hirofumi Narita, Takashi Kitahara, Yutaka Takagi, Yoshiya Sugai, Ayano Naoe, Hirayuki Sato |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Cell Extracts Male Systematic error Spectrometry Mass Electrospray Ionization Ceramide skin QD415-436 Ceramides high sensitivity Biochemistry chemistry.chemical_compound Endocrinology Lipidomics Methods Stratum corneum medicine Humans Selected ion monitoring Chromatography Fatty Acids Chromatography liquid Cell Biology Highly sensitive Forearm Cheek medicine.anatomical_structure chemistry relative response simultaneous selected ion monitoring lipids (amino acids peptides and proteins) Epidermis normal-phase liquid chromatography–electrospray ionization-mass spectrometry Chromatography Liquid |
| Zdroj: | Journal of Lipid Research, Vol 50, Iss 8, Pp 1708-1719 (2009) |
| ISSN: | 0022-2275 |
| Popis: | One of the key challenges in lipidomics is to quantify lipidomes of interest, as it is practically impossible to collect all authentic materials covering the targeted lipidomes. For diverse ceramides (CER) in human stratum corneum (SC) that play important physicochemical roles in the skin, we developed a novel method for quantification of the overall CER species by improving our previously reported profiling technique using normal-phase liquid chromatography-electrospray ionization-mass spectrometry (NPLC-ESI-MS). The use of simultaneous selected ion monitoring measurement of as many as 182 kinds of molecular-related ions enables the highly sensitive detection of the overall CER species, as they can be analyzed in only one SC-stripped tape as small as 5 mm × 10 mm. To comprehensively quantify CERs, including those not available as authentic species, we designed a procedure to estimate their levels using relative responses of representative authentic species covering the species targeted, considering the systematic error based on intra-/inter-day analyses. The CER levels obtained by this method were comparable to those determined by conventional thin-layer chromatography (TLC), which guarantees the validity of this method. This method opens lipidomics approaches for CERs in the SC.—Masukawa, Y., H. Narita, H. Sato, A. Naoe, N. Kondo, Y. Sugai, T. Oba, R. Homma, J. Ishikawa, Y. Takagi, and T. Kitahara. Comprehensive quantification of ceramide species in human stratum corneum. J. Lipid. Res. 2009. 50: 1708–1719. |
| Databáze: | OpenAIRE |
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