Tissue-selective effects of injected deoxycholate
| Autor: | Mahmood Nadir Ahmeduddin, Jason J. Bentow, Kristeene Knopp, Rattapon Thuangtong, David Nathaniel E, Michael S. Kolodney |
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| Rok vydání: | 2010 |
| Předmět: |
Keratinocytes
Cholagogues and Choleretics Lysis Injections Subcutaneous Cell Cell Culture Techniques Subcutaneous Fat Adipose tissue Mice Obese Dermatology chemistry.chemical_compound Mice In vivo Adipocyte Adipocytes Medicine Animals Humans Viability assay Muscle Skeletal Skin Muscle Cells business.industry General Medicine Fibroblasts In vitro Cell biology medicine.anatomical_structure chemistry Cell culture Immunology Models Animal Surgery business Deoxycholic Acid |
| Zdroj: | Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]. 36(6) |
| ISSN: | 1524-4725 |
| Popis: | BACKGROUND Recent studies suggest that the principal active ingredient in phosphatidylcholine-containing injectable fat-reduction formulations is actually deoxycholate (DC). This bile acid acts as a detergent to rapidly disrupt cell membranes. Thus, it is not obvious why DC would preferentially target fat. OBJECTIVE To investigate possible mechanisms for the selectivity of DC for fat tissue using in vivo and in vitro models. METHODS AND MATERIALS Histology, drug distribution studies, and cell viability assays were used to examine possible mechanisms contributing to DC selectivity. RESULTS In vitro, DC caused the lysis of all cell types tested within the tested concentration range. DC injected into fat tissue caused adipocyte death, whereas other cell types appeared less affected. Physiological concentrations of albumin or protein-rich tissues decrease the ability of DC to lyse cells. Furthermore, DC relocated to the gastrointestinal tract in animals within hours of injection. This suggests that similar mechanisms may be present in humans. CONCLUSION We report observations that provide a possible explanation for the in vivo preferential fat targeting by DC. Fat tissue, being deficient in cell-associated proteins and interstitial albumin, may be unable to sufficiently neutralize the detergent activity of DC, possibly making fat uniquely sensitive to DC. |
| Databáze: | OpenAIRE |
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