Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Autor: Ying Luo, Liangyi Chen, Kai Wang, Chengsheng Han, Xi Wang
Rok vydání: 2017
Předmět:
Male
0301 basic medicine
endocrine system
Scaffold
medicine.medical_specialty
endocrine system diseases
General Chemical Engineering
Islets of Langerhans Transplantation
Bioengineering
Streptozocin
General Biochemistry
Genetics and Molecular Biology
Diabetes Mellitus
Experimental
Andrology
Mice
03 medical and health sciences
Diabetes mellitus
Internal medicine
medicine
Animals
Whole blood
Epididymis
geography
Type 1 diabetes
geography.geographical_feature_category
General Immunology and Microbiology
business.industry
General Neuroscience
Diabetic mouse
Islet
medicine.disease
Streptozotocin
Mice
Inbred C57BL
Transplantation
Disease Models
Animal
Diabetes Mellitus
Type 1
Treatment Outcome
surgical procedures
operative
030104 developmental biology
Endocrinology
Adipose Tissue
Hyperglycemia
business
medicine.drug
Zdroj: Journal of Visualized Experiments.
ISSN: 1940-087X
Popis: Islet transplantation has been clinically proven to be effective at treating type 1 diabetes. However, the current intrahepatic transplantation strategy may incur acute whole blood reactions and result in poor islet engraftment. Here, we report a robust protocol for the transplantation of islets at the extrahepatic transplantation site-the epididymal fat pad (EFP)-in a diabetic mouse model. A protocol to isolate and purify islets at high yields from C57BL/6J mice is described, as well as a transplantation method performed by seeding islets onto a decellularized scaffold (DCS) and implanting them at the EFP site in syngeneic C57BL/6J mice rendered diabetic by streptozotocin. The DCS graft containing 500 islets reversed the hyperglycemic condition within 10 days, while the free islets without DCS required at least 30 days. The normoglycemia was maintained for up to 3 months until the graft was explanted. In conclusion, DCS enhanced the engraftment of islets into the extrahepatic site of the EFP, which could easily be retrieved and might provide a reproducible and useful platform for investigating the scaffold materials, as well as other transplantation parameters required for a successful islet engraftment.
Databáze: OpenAIRE
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