Physiological and transcriptome analysis of Poa pratensis var. anceps cv. Qinghai in response to cold stress
| Autor: | Xiang Ma, Chunxu Zhao, Wenke Dong, Hanyu Jiang, Huiling Ma |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Science Biology Phenotypic and physiological changes 01 natural sciences Citric Acid Transcriptome 03 medical and health sciences Gene Expression Regulation Plant lcsh:Botany Botany Glycolysis Poa pratensis var. anceps cv. Qinghai RNA-Seq KEGG Gene Poa Poa pratensis Phenylpropanoid Cold-Shock Response Gene Expression Profiling Molecular Sequence Annotation biology.organism_classification lcsh:QK1-989 Citric acid cycle 030104 developmental biology Gene Ontology Phenotype Gluconeogenesis Differentially expressed genes Cold stress Genome Plant 010606 plant biology & botany Research Article |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 20, Iss 1, Pp 1-18 (2020) |
| ISSN: | 1471-2229 |
| Popis: | Background Low temperature limits the growth and development and geographical distribution of plants. Poa pratensis is a cool-season turfgrass mainly grown in urban areas. However, low winter temperature or cold events in spring and autumn may cause P.pratensis mortality, affecting the appearance of lawns. P.pratensis var. anceps cv. Qinghai (PQ) is widely distributed in the Qinghai-Tibet Plateau above 3000 m. PQ has greater cold tolerance than the commercially cultivated P.pratensis varieties. However, existing studies on the response mechanism of PQ to low temperatures have mainly focused on physiological and biochemical perspectives, while changes in the PQ transcriptome during the response to cold stress have not been reported. Results To investigate the molecular mechanism of the PQ cold response and identify genes to improve the low-temperature tolerance of P.pratensis, we analyzed and compared the transcriptomes of PQ and the cold-sensitive P.pratensis cv. ‘Baron’ (PB) under cold stress using RNA sequencing. We identified 5996 and 3285 differentially expressed genes (DEGs) between the treatment vs control comparison of PQ and PB, respectively, with 5612 DEGs specific to PQ. Based on the DEGs, important Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, such as “starch and sucrose metabolism”, “protein processing in endoplasmic reticulum”, “phenylalanine metabolism” and “glycolysis/gluconeogenesis” were significantly enriched in PQ, and “starch and sucrose metabolism”, “phenylpropanoid biosynthesis”, “galactose metabolism” and “glutathione metabolism” were significantly enriched in PB. In addition, the “glycolysis” and “citrate cycle (TCA cycle)” pathways were identified as involved in cold tolerance of P.pratensis. Conclusions As we know, this is the first study to explore the transcriptome of P.pratensis var. anceps cv. Qinghai. Our study not noly provides important insights into the molecular mechanisms of P.pratensis var. anceps cv. Qinghai responds to cold stress, but also systematically reveals the changes of key genes and products of glycolysis and TCA cycle in response to cold stress, which is conductive to the breeding of cold-tolerance P.pratensis genotype. |
| Databáze: | OpenAIRE |
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