Ethnic differences in the expression of Epstein-Barr virus latent membrane protein-1 mutations in nasopharyngeal carcinoma
| Autor: | Peter J. Chauvin, M D’Addario |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Herpesvirus 4
Human Viral protein Health Toxicology and Mutagenesis DNA Mutational Analysis Molecular Sequence Data Biology medicine.disease_cause Polymerase Chain Reaction Virus Herpesviridae White People Viral Matrix Proteins Asian People otorhinolaryngologic diseases Genetics medicine Ethnicity Humans Molecular Biology DNA Primers Mutation Base Sequence Nasopharyngeal Neoplasms Epstein–Barr virus latent membrane protein 1 medicine.disease Virology Epstein–Barr virus stomatognathic diseases Nasopharyngeal carcinoma Inuit DNA Viral Carcinogenesis |
| Zdroj: | Mutation research. 457(1-2) |
| ISSN: | 0027-5107 |
| Popis: | The latent membrane protein-1 (LMP1) of Epstein-Barr virus (EBV) is a viral oncoprotein implicated in several EBV-associated pathologies. Many studies have characterized carboxy-terminal mutations within LMP1, errors in this area are critical since this portion contains sequences responsible for LMP1 targeting, half-life and association with host cell proteins. Although, data suggests that mutations in this area extend LMP1 half-life and increase its oncogenesis, some studies have not shown this to be true for all EBV-associated tumors. In order to evaluate 3'-end LMP1-DNA mutations in three different ethnic populations with nasopharyngeal carcinoma (NPC), we examined EBV-DNA in 34 patients of various origins (Caucasian, Chinese and Inuit). While 68% of the total group expressed EBV-antigens, only 56% of Caucasians but 86% of Inuit expressed this viral protein. Over 67% of Inuit NPC tissue contained the characteristic 30 bp deletion that was observed in only 20% of Caucasians and 33% of Chinese samples. DNA sequencing revealed that the Inuit population showed the most frequent DNA mutations and corresponding amino acid alterations in LMP1. Our results suggest that EBV-associated NPC-DNA mutations in LMP1 do not occur at equal rates in different racial groups and are more common at distinct sites in NPC tissue from Chinese and Inuit sources. |
| Databáze: | OpenAIRE |
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