Babesia Canis Canis, Babesia Canis Vogeli, Babesia Canis Rossi: Differentiation of the Three Subspecies By A Restriction Fragment Length Polymorphism Analysis On Amplified Small Subunit Ribosomal Rna Genes
| Autor: | Céline Carret, Nathalie Grande, André Gorenflot, Theo Schetters, B. Carcy, K. Moubri, Fabien Walas, Eric Precigout |
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| Rok vydání: | 1999 |
| Předmět: |
Molecular Sequence Data
Babesia Biology Subspecies DNA Ribosomal Polymerase Chain Reaction Microbiology Dogs Babesiosis Cricetinae parasitic diseases medicine Animals Humans Dog Diseases Babesia divergens Phylogeny Genetics Genes rRNA Sequence Analysis DNA DNA Protozoan Ribosomal RNA biology.organism_classification medicine.disease Canis Babesia canis Cattle Restriction fragment length polymorphism Polymorphism Restriction Fragment Length |
| Zdroj: | The Journal of Eukaryotic Microbiology. 46:298-301 |
| ISSN: | 1550-7408 1066-5234 |
| DOI: | 10.1111/j.1550-7408.1999.tb05128.x |
| Popis: | The parasites Babesia canis and Babesia gibsoni (phylum Apicomplexa) are responsible for canine babesiosis throughout the world. Babesia canis was previously described as a group of three biologically different subspecies, namely B. canis canis, B. canis vogeli, and B. canis rossi. We report partial sequences of small subunit ribosomal RNA gene (ssu-rDNA) of each subspecies amplified in vitro with primers derived from a semi-conserved region of the ssu-rDNA genes in other Babesia species. The polymerase chain reaction combined with a restriction fragment length polymorphism analysis, using HinfI and TaqI restriction enzymes, confirmed the separation of B. canis into three subspecies. These sequences were compared with previously published sequences of other Babesia species. A phylogenetic approach showed that the three subspecies of B. canis belong to the clade of Babesia species sensu stricto where B. canis canis clusters with B. canis rossi whereas B. canis vogeli might form a monophyletic group with the cluster B. divergens and B. odocoilei. Our results show that the three subspecies of B. canis can readily be differentiated at the molecular level and suggest that they might be considered as true species. |
| Databáze: | OpenAIRE |
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