Polycyclic aromatic hydrocarbon-emulsifier protein produced by Aspergillus brasiliensis (niger) in an airlift bioreactor following an electrochemical pretreatment
Autor: | Ignacio González, Mariano Gutiérrez-Rojas, Keiko Shirai, Victor Sánchez-Vázquez |
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Rok vydání: | 2018 |
Předmět: |
0106 biological sciences
0301 basic medicine Environmental Engineering Polycyclic aromatic hydrocarbon Salt (chemistry) Bioengineering 01 natural sciences 03 medical and health sciences Bioreactors Bioremediation 010608 biotechnology Bioreactor Niger Polycyclic Aromatic Hydrocarbons Waste Management and Disposal chemistry.chemical_classification Aspergillus Chromatography biology Renewable Energy Sustainability and the Environment Airlift Substrate (chemistry) General Medicine biology.organism_classification 030104 developmental biology chemistry Emulsifying Agents Emulsion Aspergillus niger |
Zdroj: | Bioresource Technology. 256:408-413 |
ISSN: | 0960-8524 |
Popis: | An emulsifier protein (EP) was produced and easily separated from oil-contaminated water as an economical substrate when Aspergillus brasiliensis, pretreated in a solid state culture with a controlled electric field, was used in an airlift bioreactor. The hydrocarbon-EP comprised 19.5% of the total protein, its purification enhanced the specific emulsifying activity (EA) seven times. The influence of operational conditions (pH and salt concentration) on the EA were assessed to characterise the emulsion stability. The EA was increased by 19% in alkaline environments (pH 7–11), but it was not affected by the presence of salt (0–35 g L−1). On the other hand, preheating the EP samples (60 °C) enhanced the EA by 2.5 times. Based on analysis of its EA, this EP can be applied as a bioremediation enhancer in contaminated soils. |
Databáze: | OpenAIRE |
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