Chronic regulation of transepithelial Na+ transport by the rate of apical Na+ entry
| Autor: | Mark L. Zeidel, E. Lechman, E. Sarac, John P. Johnson, J. Angeski, Michael West, Michael D. Rokaw |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Time Factors Physiology ATPase Sodium chemistry.chemical_element Kidney Epithelium Sodium Channels Amiloride chemistry.chemical_compound Xenopus laevis Internal medicine medicine Animals Ion transporter Epithelial polarity Cell Line Transformed Tetramethylammonium biology Sodium channel Cell Membrane Biological Transport Cell Biology Intracellular Membranes Apical membrane Quaternary Ammonium Compounds Endocrinology chemistry biology.protein Biophysics Sodium-Potassium-Exchanging ATPase medicine.drug |
| Zdroj: | The American journal of physiology. 270(2 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | In several settings in vivo, prolonged inhibition of apical Na+ entry reduces and prolonged stimulation of apical entry enhances the ability of renal epithelial cells to reabsorb Na+, an important feature of the load-dependent regulation of renal tubular Na+ transport. To model this load dependency, apical Na+ entry was inhibited or stimulated for 18 h in A6 cells and vectorial transport was measured as short-circuit current (Isc) across monolayers on filter-bottom structures. Basal amiloride-sensitive Isc represents the activity of apical Na+ channels, whereas Isc after permeabilization of the apical membrane to cations with nystatin represents maximal activity of the basolateral Na(+)-K(+)-ATPase. Chronic inhibition of apical Na+ entry by 18-h apical exposure to amiloride or replacement of apical Na+ with tetramethylammonium (TMA+), followed by washing and restoration of normal apical medium, revealed a persistent decrease in Isc that remained despite exposure to nystatin. Both basal and nystatin-stimulated Isc recovered progressively after restoration of normal apical medium. In contrast, chronic stimulation of apical Na+ entry by short circuiting the epithelium increased Isc in the absence and presence of nystatin, indicating upregulation of both apical Na+ channels and basolateral Na(+)-K(+)-ATPase. Basolateral equilibrium [3H]ouabain binding was reduced to 67 +/- 5% in TMA+ vs. control cells, whereas values in 18-h short-circuited cells increased by 42 +/- 19%. The results demonstrate that load dependency of tubular Na+ transport can be modeled in vitro and indicate that the regulation of Na(+)-K(+)-ATPase observed in these studies occurs in part by changes in the density of functional transporter proteins within the basolateral membrane. |
| Databáze: | OpenAIRE |
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