Persistence of Klebsiella pneumoniae ST258 as the predominant clone of carbapenemase-producing Enterobacteriaceae in post-acute-care hospitals in Israel, 2008-13
| Autor: | Adler, A., Hussein, O., Ben-David, D., Masarwa, S., Navon-Venezia, S., Schwaber, M. J., Carmeli, Y., Setton, E., Golan, S., Brill, S., Lipkin, V., Frodin, E., Mendelson, G., Rave, R., Yehuda, N., Aizen, I., Kaganski, M., Gershkovich, P., Sasson, A., Yosef, H., Stessman, J., Zlatkin, S., Or, I., Lazary, A., Weinberg, I., Madjar, J., Taichman, S., Ben-Israel, J., Vigder, C., Bar'el, C., Davidovitch, Y., Charish, L. |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
clone (Java method) Genotype Klebsiella pneumoniae Population Prevalence beta-Lactamases Microbiology Bacterial Proteins polycyclic compounds Humans Pharmacology (medical) Israel education Retrospective Studies Pharmacology Cross Infection Molecular Epidemiology education.field_of_study biology Molecular epidemiology Enterobacteriaceae Infections biochemical phenomena metabolism and nutrition biology.organism_classification Hospitals Molecular Typing Cross-Sectional Studies Infectious Diseases Carriage Multilocus sequence typing |
| Zdroj: | Journal of Antimicrobial Chemotherapy |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dku333 |
| Popis: | Objectives To study the molecular characteristics of carbapenemase-producing Enterobacteriaceae (CPE) in post-acute-care hospitals (PACHs) in Israel and to analyse the temporal changes between 2008 and 2013. Methods CPE isolates were obtained during two cross-sectional, point prevalence national surveys of PACHs in Israel performed in 2008 and 2013. Surveillance cultures were collected by streaking rectal swabs onto selective media. Isolates were identified to species level and tested for blaKPC, blaNDM and blaOXA-48 by PCR and by the Carba NP test. Molecular typing was done by PCR for the pilv-l gene, designed for the ST258 KPC-producing Klebsiella pneumoniae (KPC-KP) clone, BOX-PCR and MLST. Results The prevalence of CPE carriage in the first survey was 184/1147 (16%); all of the isolates were KPC-KP. The prevalence of CPE carriage in the second survey was 127/1287 (9.9%); of these isolates, 113 (89%) were KPC-KP, 9 (7%) were other KPC-producing species and 5 (4%) were NDM- and OXA-48-producing CPE (n = 1 and 4, respectively). The proportion of the KPC-KP population represented by the ST258 clone increased from 120/184 (65%) in 2008 to 91/113 (80%) in 2013. In 58% (71/122) of the KPC-CPE carriers identified in the 2013 survey, the source of acquisition was determined to be the PACH itself. All four OXA-48 CPE were acquired either directly or indirectly from patients arriving from the Palestinian Authority or Syria. Conclusions Despite the decreased prevalence of CPE in Israeli PACHs, and the emergence of new types of CPE, the KPC-KP ST258 clone remains the predominant clone represented. |
| Databáze: | OpenAIRE |
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