A unique bacteriohopanetetrol stereoisomer of marine anammox

Autor: Darci Rush, E. Alex Charlton, Philippe Schaeffer, Helen M. Talbot, Philippe Normand, D. Martin Jones, Rachel Schwartz-Narbonne, Jaap S. Sinninghe Damsté, Mike S. M. Jetten, Margot Schenesse, Guylaine H. L. Nuijten, Ellen C. Hopmans, Muhammad Farhan Ul Haque, J. Colin Murrell, Sabine K. Lengger
Přispěvatelé: Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Newcastle University [Newcastle], Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Utrecht University [Utrecht], Royal Netherlands Institute for Sea Research (NIOZ), University of East Anglia [Norwich] (UEA), University of the Punjab, Radboud university [Nijmegen], School of Geography, Earth and Environmental Sciences [Plymouth] (SoGEES), Plymouth University, School of Environmental Sciences [Norwich], Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Departments of Archaeology [York] (BioArch), University of York [York, UK], Natural Environment Research Council (NERC), United Kingdom project ANAMMARKS NE/N011112/1SIAM, Netherlands 024002002EAOG, Netherlands Research Award Rubicon fellowship from the Netherlands Organization for Scientific Research (NWO), Netherlands 825.14.014European Research Council (ERC) European Union under the European Union's Horizon 2020 research and innovation program 694569 -MICROLIPIDSLeverhulme TrustRPG2016-050
Jazyk: angličtina
Rok vydání: 2020
Předmět:
bepress|Physical Sciences and Mathematics
Komagataeibacter xylinus
Bacteriohopanetetrols
010504 meteorology & atmospheric sciences
bepress|Physical Sciences and Mathematics|Earth Sciences
EarthArXiv|Physical Sciences and Mathematics|Earth Sciences
010502 geochemistry & geophysics
01 natural sciences
High-performance liquid chromatography
Anammox
Geochemistry and Petrology
[SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry
Bacteriohopanepolyols
Stereoisomers
14. Life underwater
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
0105 earth and related environmental sciences
Chromatography
Bacteriohopanetetrol
biology
Chemistry
Anaerobic ammonium oxidation
biology.organism_classification
EarthArXiv|Physical Sciences and Mathematics
Biomarker (petroleum)
EarthArXiv|Physical Sciences and Mathematics|Earth Sciences|Biogeochemistry
13. Climate action
Ecological Microbiology
[SDE]Environmental Sciences
Scalindua
bepress|Physical Sciences and Mathematics|Earth Sciences|Biogeochemistry
Bacteria
Biomarkers
Zdroj: Organic Geochemistry
Organic Geochemistry, Elsevier, 2020, 143, pp.103994. ⟨10.1016/j.orggeochem.2020.103994⟩
Organic Geochemistry, 143
ISSN: 0146-6380
DOI: 10.1016/j.orggeochem.2020.103994⟩
Popis: Anaerobic ammonium oxidation (anammox) is a significant process for bioavailable nitrogen removal from marine systems. A bacteriohopanetetrol (BHT) isomer, with unknown stereochemistry, eluting later than BHT using high performance liquid chromatography (HPLC), was detected in ‘Ca. Scalindua profunda’ and proposed as a biomarker for anammox in marine paleo-environments. Four non-marine, non-anammox genera also produce late-eluting BHT stereoisomers. Of these, the stereochemistry in Acetobacter pasteurianus, Komagataeibacter xylinus and Frankia sp. was shown to be 17β, 21β(H), 22R, 32R, 33R, 34R (BHT-34R), while the stereochemistry of the late-eluting BHT in Methylocella palustris was unknown. We studied the BHT distributions and stereochemistry of these known BHT isomer producers and of previously unscreened marine (‘Ca. Scalindua brodeae’) and freshwater (‘Ca. Brocadia sp.’) anammox species and genera using HPLC and gas chromatographic (GC) analysis of acetylated BHTs and ultra high performance liquid chromatography (UHPLC)-High Resolution Mass Spectrometry (HRMS) analysis of non-acetylated BHTs. The 34R stereochemistry was confirmed for the BHT isomers in Ca. Brocadia sp., and Methylocella palustris. However, ‘Ca. Scalindua sp.’ synthesise a stereochemically distinct BHT isomer, with a still unconfirmed stereochemistry (BHT-x). ‘Ca. Kuenenia sp.’ was confirmed not to produce any late-eluting BHT stereoisomers. Only GC analysis of acetylated BHT and UHPLC analysis of non-acetylated BHT distinguished between late-eluting BHT isomers. Acetylated BHT-x and BHT-34R co-elute by HPLC. As BHT-x is so far only known to be produced by ‘Ca. Scalindua spp.’, it may be applied as a biomarker for marine anammox. As ‘Ca. Brocadia sp.’ produces BHT-34R, this BHT isomer is a potential, albeit less specific, biomarker for anammox in non-marine settings.
Databáze: OpenAIRE
Externí odkaz: