Effect of triploidy on digestive enzyme activity of early stages of turbot (Scophthalmus maximus)
| Autor: | Pedro Gallardo, Rosa Cal, Jorge Hernández-Urcera, Ariadna Sánchez, María Eugenia Chimal, Carlos Rosas, Pedro Domingues, Alberto Olivares |
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| Přispěvatelé: | Gallardo, P., Gallardo, P. [0000-0002-1014-4564] |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
Proteases Physiology medicine.medical_treatment Growth Aquatic Science Biochemistry Gene Expression Regulation Enzymologic 03 medical and health sciences Digestive enzymes Pepsin medicine Animals Amylase 030304 developmental biology 0303 health sciences Protease biology fungi 04 agricultural and veterinary sciences General Medicine Turbot Trypsin biology.organism_classification Triploidy Enzyme assay Gastrointestinal Tract Digestive enzyme Flatfishes 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Peptide Hydrolases medicine.drug |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| Popis: | 10 pages, 4 figures In the present work, growth and digestive enzyme activities of total acid and alkaline proteases, pepsin, trypsin, lipase, and α-amylase, as well as partial characterization of enzyme activity, were studied in diploid and triploid turbot. Growth was similar between both groups. Acid protease activity increased consistently during the experiment, for both diploid (2n) and triploid (3n) fish. The alkaline protease activity was always higher for triploids throughout the experiment. Proteolytic acid activity (pH 2) was generally higher for diploids, at all temperatures tested. Higher activity was at pH 2 and 3 for 2n and 3n fish, respectively. Regarding temperature, acid and alkaline protease activity was higher at 37 °C and 60 °C, respectively, for both groups. The general increase in pancreatic enzymes (trypsin and amylase) before 35 days after hatching (DAH) and posterior decrease until 60 DAH. There was a marked effect on enzyme activity when changing from live prey to pellets (35 DAH), especially on triploids This research received funding from the project “RODAGEN” – PROPORCION DE SEXOS EN LA PROGENIE DE RODABALLO from the Spanish government and also from PAPIIT UNAM-IT201117 and TEMPOXMAR collaboration net financed by DGECI-UNAM |
| Databáze: | OpenAIRE |
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