Functional Characterization of Cysteinyl Leukotriene CysLT2 Receptor on Human Coronary Artery Smooth Muscle Cells
| Autor: | Tetsu Saito, Jun Takasaki, Kiyoshi Furuichi, Masazumi Kamohara, Hitoshi Matsushime, Takatoshi Soga, Shunichiro Matsumoto, Mitsuyuki Matsumoto |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.medical_specialty
Vascular smooth muscle Biophysics In situ hybridization Biology Biochemistry Muscle Smooth Vascular Calcium in biology Cardiovascular Physiological Phenomena Internal medicine medicine Humans Myocyte Tissue Distribution Receptor Molecular Biology Receptors Leukotriene Chemotaxis Membrane Proteins Biological activity Arteries Cell Biology Anatomy respiratory system Coronary Vessels Leukotriene C4 medicine.anatomical_structure Endocrinology lipids (amino acids peptides and proteins) Artery |
| Zdroj: | Biochemical and Biophysical Research Communications. 287:1088-1092 |
| ISSN: | 0006-291X |
| Popis: | Cysteinyl leukotrienes (LTC(4), LTD(4), and LTE(4)) are a class of biologically active lipids that exert potent effects on the heart. To assess their roles, we investigated the distribution of their receptors, CysLT(1) and CysLT(2), in the cardiovascular system. CysLT(2) mRNA was detected at high levels in the human atrium and ventricle and at intermediate levels in the coronary artery, whereas CysLT(1) mRNA was barely detected. Further analysis by in situ hybridization revealed that CysLT(2) mRNA was expressed in myocytes, fibroblasts, and vascular smooth muscle cells, but not in endothelial cells. When human coronary smooth muscle cells were stimulated with LTC(4), the intracellular calcium concentration increased in a dose-dependent manner, and this action was partially inhibited by nicardipine. Additionally, these cells showed chemotactic responses to LTC(4). This is the first report on the physiological role of CysLT(2), and the findings suggest that CysLT(2) has biological significance in the cardiovascular system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect