| Popis: |
CRISPR/Cas9 technology, a genetic modification tool developed from certain bacteria's defence mechanism against viruses and plasmids, brought genome editing back into the public eye in 2012. This approach is simple to employ and has been tested on a range of experimental models, including cell lines, animals in the lab, plants, and even humans in clinical trials. The Cas9 nuclease is instructed to make a site- directed double-strand DNA break utilising the short RNA molecule as a guide in the CRISPR/Cas9 system. This technology has advanced significantly in recent years, and it is now often used to edit the genome to add, remove, or modify certain genes. Additionally, CRISPR/Cas9-based gene drive may have the ability to stop the spread of vector-borne illnesses. Although comprehensive testing is necessary to support these claims, the bacterial and archaeal adaptive immune systems may offer a therapeutic solution to previously incurable illnesses. using regard to disease modelling and therapy, we discuss current advances made using CRISPR/Cas9 in this review, as well as what future considerations should be made when utilising this technology. |
