Comparison of internal energy distributions generated by supercritical fluid chromatography versus liquid chromatography hyphenated with electrospray high resolution mass spectrometry

Autor: David Touboul, Thi Phuong Thuy Hoang
Přispěvatelé: Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Electrospray
Capillary action
Electrospray ionization
010402 general chemistry
01 natural sciences
Biochemistry
Chemistry Techniques
Analytical
Mass Spectrometry
Analytical Chemistry
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
Phase (matter)
ComputingMilieux_MISCELLANEOUS
Chromatography
Internal energy
Chemistry
010401 analytical chemistry
Organic Chemistry
Chromatography
Supercritical Fluid
General Medicine
Supercritical fluid
0104 chemical sciences
Volumetric flow rate
[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry
Supercritical fluid chromatography
Solvents
[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
[CHIM.OTHE]Chemical Sciences/Other
[CHIM.CHEM]Chemical Sciences/Cheminformatics
Chromatography
Liquid
Zdroj: Journal of Chromatography A
Journal of Chromatography A, Elsevier, 2020, 1634 (11), pp.461703. ⟨10.1016/j.chroma.2020.461703⟩
ISSN: 0021-9673
1873-3778
DOI: 10.1016/j.chroma.2020.461703⟩
Popis: The internal energy distributions of thermometer ions dissolved in supercritical and liquid solvents and produced in the gas phase by electrospray (ESI) were measured and compared by the survival yield method. The influence of different chromatographic conditions such as the nature of solvents, the composition of the mobile phase, the pressure of the back pressure regulator was studied for supercritical fluid chromatography (SFC) whereas the influence of the composition and of the flow rate of the mobile phase was investigated for liquid chromatography (LC). The MS instrumental parameters were studied in parallel for SFC and LC showing that the drying gas temperature and the fragmentor voltage affected the internal energy distribution, whereas the capillary voltage did not modify the internal energy distribution. A comparison of the internal energy distributions generated by SFC and LC was carried out leading to conclude that SFC led to higher internal energy, i.e. more in-source fragment ions, than LC.
Databáze: OpenAIRE
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