Short Peptides Enhance Single Cell Adhesion and Viability on Microarrays
| Autor: | Omid Veiseh, Fareid Asphahani, Mandana Veiseh, Miqin Zhang, Michael C. Martin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
Cell Survival
Surface Properties Peptide Article Substrate Specificity Tissue engineering Cell Adhesion Electrochemistry Humans General Materials Science Viability assay Cell adhesion Electrodes Spectroscopy chemistry.chemical_classification Microscopy Confocal Tissue Engineering biology Cell adhesion molecule Microarray analysis techniques Life Sciences Biosensors Cell adhesion FTIR BioMEMS self-assembled monolayers Surfaces and Interfaces Adhesion Microarray Analysis Silicon Dioxide Condensed Matter Physics Fibronectins Cell biology Fibronectin Microscopy Fluorescence Biochemistry chemistry biology.protein Endothelium Vascular Peptides |
| Zdroj: | Veiseh, Mandana; Veiseh, Omid; Martin, Michael C.; Asphahani, Fareid; & Zhang, Miqin. (2007). Short Peptides Enhance Single Cell Adhesion and Viability on Microarrays. Lawrence Berkeley National Laboratory. Lawrence Berkeley National Laboratory: Lawrence Berkeley National Laboratory. Retrieved from: http://www.escholarship.org/uc/item/3cv896gz |
| Popis: | Single cell patterning holds important implications for biology, biochemistry, biotechnology, medicine, and bioinformatics. The challenge for single cell patterning is to produce small islands hosting only single cells and retaining their viability for a prolonged period of time. This study demonstrated a surface engineering approach that uses a covalently bound short peptide as a mediator to pattern cells with improved single cell adhesion and prolonged cellular viability on gold patterned SiO2 substrates. The underlying hypothesis is that cell adhesion is regulated bythe type, availability, and stability of effective cell adhesion peptides, and thus covalently bound short peptides would promote cell spreading and, thus, single cell adhesion and viability. The effectiveness of this approach and the underlying mechanism for the increased probability of single cell adhesion and prolonged cell viability by short peptides were studied by comparing cellular behavior of human umbilical cord vein endothelial cells on three modelsurfaces whose gold electrodes were immobilized with fibronectin, physically adsorbed Arg-Glu-Asp-Val-Tyr, and covalently bound Lys-Arg-Glu-Asp-Val-Tyr, respectively. The surface chemistry and binding properties were characterized by reflectance Fourier transform infrared spectroscopy. Both short peptides were superior to fibronectin in producing adhesion of only single cells, whereas the covalently bound peptide also reduced apoptosis and necrosisof adhered cells. Controlling cell spreading by peptide binding domains to regulate apoptosis and viability represents a fundamental mechanism in cell-materials interaction and provides an effective strategy in engineering arrays of single cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|