Gastric Cancer Extracellular Vesicles Tune the Migration and Invasion of Epithelial and Mesenchymal Cells in a Histotype-Dependent Manner

Autor: Joana Carvalho, Sara Pinto Teles, Carla Oliveira, Patrícia Oliveira, Mafalda Azevedo, Sara Rocha
Přispěvatelé: Instituto de Investigação e Inovação em Saúde
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Fibronectins / metabolism
migration
Metastasis
lcsh:Chemistry
0302 clinical medicine
Invasion
Cell Movement
Stomach Neoplasms / metabolism
lcsh:QH301-705.5
Spectroscopy
Migration
Cells
Cultured
0303 health sciences
biology
Chemistry
Epithelial Cells / physiology
General Medicine
Fibronectins / genetics
Extracellular vesicles
invasion
Mesenchymal Stem Cells / metabolism
3. Good health
Computer Science Applications
Cell biology
030220 oncology & carcinogenesis
epithelial-to-mesenchymal transition
extracellular vesicles
Epithelial Cells / metabolism
Signal Transduction
Extracellular Vesicles / metabolism
Epithelial-Mesenchymal Transition
Mesenchymal Stem Cells / physiology
Epithelial Cells / pathology
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Stomach Neoplasms
Cell Line
Tumor
medicine
Humans
Epithelial–mesenchymal transition
Physical and Theoretical Chemistry
Molecular Biology
030304 developmental biology
Differential centrifugation
Tumor microenvironment
gastric cancer
Organic Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells / pathology
Cancer
Epithelial Cells
Mesenchymal Stem Cells
medicine.disease
Fibronectins
Fibronectin
Epithelial-to-mesenchymal transition
lcsh:Biology (General)
lcsh:QD1-999
Cancer cell
biology.protein
Gastric cancer
Zdroj: International Journal of Molecular Sciences, Vol 20, Iss 11, p 2608 (2019)
International Journal of Molecular Sciences
Volume 20
Issue 11
ISSN: 1422-0067
Popis: Extracellular vesicles (EVs) secreted by tumor cells modulate recipient cells&rsquo
behavior, but their effects in normal cells from the tumor microenvironment remain poorly known. In this study, we dissected the functional impact of gastric cancer cell-derived EVs (GC-EVs), representative of distinct GC histotypes, on the behavior of normal isogenic epithelial and mesenchymal cells. GC-EVs were isolated by differential centrifugation and characterized by transmission electron microscopy, nanoparticle tracking analysis, and imaging flow-cytometry. Epithelial and mesenchymal cells were challenged with GC-EVs and submitted to proliferation, migration, and invasion assays. Expression of epithelial and mesenchymal markers was followed by immunofluorescence and flow-cytometry. Our results indicated that GC-EVs secreted by diffuse-type cancer cells decrease the migration of recipient cells. This effect was more prominent and persistent for mesenchymal recipient cells, which also increased Fibronectin expression in response to EVs. GC-EVs secreted by cancer cells derived from tumors with an intestinal component increased invasion of recipient epithelial cells, without changes in EMT markers. In summary, this study demonstrated that GC-EVs modulate the migration and invasion of epithelial and mesenchymal cells from the tumor microenvironment, in a histotype-dependent manner, highlighting new features of intestinal and diffuse-type GC cells, which may help explaining differential metastasis patterns and aggressiveness of GC histotypes.
Databáze: OpenAIRE
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