Adsorption of ethylenediaminetetraacetic dianhydride modified oxalate decarboxylase on calcium oxalate

Autor:	Rihui Lin, Jia Wu, XY Wang, YC Wu, Junbin He, Xinghua Cai
Rok vydání:	2018
Předmět:	Histology Calcium Oxalate Carboxy-Lyases 030232 urology & nephrology Calcium oxalate Ethylenediaminetetraacetic acid General Medicine 010402 general chemistry 01 natural sciences 0104 chemical sciences Oxalate decarboxylase Quaternary Ammonium Compounds 03 medical and health sciences Medical Laboratory Technology chemistry.chemical_compound 0302 clinical medicine Adsorption chemistry Nuclear chemistry
Zdroj:	Biotechnichistochemistry : official publication of the Biological Stain Commission. 93(3)
ISSN:	1473-7760
Popis:	We used ethylenediaminetetraacetic acid dianhydride (EDTAD) to modify oxalate decarboxylase (OXDC) to improve its adsorption on calcium oxalate stones. The modified sites were identified by Ultra performance liquid chromatography-mass spectrometry (UPLC-MS) and the adsorption mechanism of the EDTAD-modified OXDC on calcium oxalate (CaOx) was investigated. We investigated adsorption time, initial enzyme concentration, temperature and solution pH on the adsorption process. Data were analyzed using kinetics, thermodynamics and isotherm adsorption models. UPLC-MS showed that EDTAD was attached to OXDC covalently and suggested that the chemical modification occurred at both the free amino of the side chain and the α-NH
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::020fa8288d67c6930994ce78a69be7b2 https://pubmed.ncbi.nlm.nih.gov/29393700 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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