Neutrophil differentiation into a unique hybrid population exhibiting dual phenotype and functionality of neutrophils and dendritic cells
| Autor: | Nobuyasu Mayuzumi, Paul Kotol, Richard L. Gallo, Shuo Geng, Hironori Matsushima, Ran Lu, Benjamin J. Chojnacki, Yi Yao, Takashi Okamoto, Akira Takashima, Toru Miyazaki |
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| Rok vydání: | 2013 |
| Předmět: |
Podosome
Neutrophils Blotting Western Immunology Antigen presentation Population Plenary Paper Antigen-Presenting Cells CD11c Hybrid Cells Biology Biochemistry Mice Phagocytosis Neutrophil differentiation Animals Antigen-presenting cell education Cells Cultured Oligonucleotide Array Sequence Analysis Mice Knockout Antigen Presentation education.field_of_study MHC class II Gene Expression Profiling Cell Differentiation Dendritic Cells Cell Biology Hematology Neutrophil extracellular traps Flow Cytometry Molecular biology Cell biology Mice Inbred C57BL Phenotype biology.protein Cytokines Biomarkers |
| Zdroj: | Blood. 121:1677-1689 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2012-07-445189 |
| Popis: | Neutrophils have been reported to acquire surface expression of MHC class II and co-stimulatory molecules as well as T-cell stimulatory activities when cultured with selected cytokines. However, cellular identity of those unusual neutrophils showing antigen presenting cell (APC)-like features still remains elusive. Here we show that both immature and mature neutrophils purified from mouse bone marrow differentiate into a previously unrecognized "hybrid" population showing dual properties of both neutrophils and dendritic cells (DCs) when cultured with granulocyte macrophage-colony-stimulating factor but not with other tested growth factors. The resulting hybrid cells express markers of both neutrophils (Ly6G, CXCR2, and 7/4) and DCs (CD11c, MHC II, CD80, and CD86). They also exhibit several properties typically reserved for DCs, including dendritic morphology, probing motion, podosome formation, production of interleukin-12 and other cytokines, and presentation of various forms of foreign protein antigens to naïve CD4 T cells. Importantly, they retain intrinsic abilities of neutrophils to capture exogenous material, extrude neutrophil extracellular traps, and kill bacteria via cathelicidin production. Not only do our results reinforce the notion that neutrophils can acquire APC-like properties, they also unveil a unique differentiation pathway of neutrophils into neutrophil-DC hybrids that can participate in both innate and adaptive immune responses. |
| Databáze: | OpenAIRE |
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