Identification and Quantification of Ricin Toxin in Animal Tissues using ELISA
| Autor: | G. D. Griffiths, D.J. Gee, Helen Newman |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Toxin
Immunocytochemistry Enzyme-Linked Immunosorbent Assay Rats Inbred Strains Radioimmunoassay Ricin Biology medicine.disease_cause Molecular biology Rats Pathology and Forensic Medicine Iodine Radioisotopes chemistry.chemical_compound Ricin toxin chemistry Injection site medicine biology.protein Animals Female Tissue Distribution Antibody Intramuscular injection |
| Zdroj: | Journal of the Forensic Science Society. 26:349-358 |
| ISSN: | 0015-7368 |
| Popis: | The purpose of this study was to provide a rapid, relatively inexpensive, reliable and straightforward method for the demonstration and quantification of ricin in animal tissues following an intramuscular injection. The use of radioiodinated ricin provided an indication as to where the highest levels of toxin might be found; the highly sensitive and specific use of antibodies in Enzyme Linked Immunosorbent Assays (ELISA) was employed as an alternative to the more expensive and more tedious procedure of radioimmunoassay. The injection site, lymphoid tissues and liver were found to contain detectable amounts of the toxin. In cases where administration of ricin were suspected, confirmation using biopsied tissue samples could enable appropriate remedial measures to be taken. Alternatively, in cases of suspicious deaths, post mortem tissues would be used to identify the presence of toxin in the body and could be of significant value to forensic diagnosis. The advantage of this method over that of immunocytochemistry is discussed. |
| Databáze: | OpenAIRE |
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