Anti-Hyperglycemic Effects of Refined Fractions from Cyclocarya paliurus Leaves on Streptozotocin-Induced Diabetic Mice
| Autor: | Cheng Chen, Zhu-Jun Fang, Chi Teng Vong, Zhe-Ling Feng, Lishe Gan, Maggie Pui Man Hoi, Ruohan Lou, Ligen Lin, Jiali Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system diseases medicine.medical_treatment Glucose uptake Cyclocarya paliurus polysaccharides Pharmaceutical Science Organic chemistry Pharmacology Polysaccharide Article Streptozocin Analytical Chemistry Cell Line Diabetes Mellitus Experimental Juglandaceae Diabetic nephropathy chemistry.chemical_compound Mice QD241-441 Drug Discovery medicine Animals Hypoglycemic Agents Physical and Theoretical Chemistry Blood urea nitrogen chemistry.chemical_classification Ethanol biology Plant Extracts anti-hyperglycemia triterpenoids medicine.disease Streptozotocin biology.organism_classification Mice Inbred C57BL Plant Leaves Cytokine chemistry Chemistry (miscellaneous) flavonoids Molecular Medicine Cyclocarya psychological phenomena and processes medicine.drug |
| Zdroj: | Molecules Molecules, Vol 26, Iss 6886, p 6886 (2021) Volume 26 Issue 22 |
| ISSN: | 1420-3049 |
| Popis: | To identify the chemical components responsible for the anti-hyperglycemic effect of Cyclocarya paliurus (Batal.) Iljinsk (Juglandaceae) leaves, an ethanol extract (CPE) and a water extract (CPW) of C. paliurus leaves, as well as their total flavonoids (CPF), triterpenoids (CPT) and crude polysaccharides (CPP), were prepared and assessed on streptozotocin (STZ)-induced diabetic mice. After being orally administrated once a day for 24 days, CPF (300 mg/kg), CPP (180 mg/kg), or CPF+CPP (300 mg/kg CPF + 180 mg/kg CPP) treatment reversed STZ-induced body weight and muscle mass losses. The glucose tolerance tests and insulin tolerance tests suggested that CPF, CPP, and CPF+CPP showed anti-hyperglycemic effect in STZ-induced diabetic mice. Furthermore, CPF enhances glucose-stimulated insulin secretion in MIN6 cells and insulin-stimulated glucose uptake in C2C12 myotubes. CPF and CPP suppressed inflammatory cytokine levels in STZ-induced diabetic mice. Additionally, CPF and CPP improved STZ-induced diabetic nephropathy assessed by H& E staining, blood urea nitrogen content, and urine creatinine level. The molecular networking and Emperor analysis results indicated that CPF showed potential anti-hyperglycemic effects, and HPLC–MS/MS analysis indicated that CPF contains 3 phenolic acids and 9 flavonoids. In contrast, CPT (650 mg/kg) and CPC (300 mg/kg CPF + 180 mg/kg CPP + 650 mg/kg CPT) did not show anti-hyperglycemic effect. Taken together, polysaccharides and flavonoids are responsible for the anti-hyperglycemic effect of C. paliurus leaves, and the clinical application of C. paliurus need to be refined. |
| Databáze: | OpenAIRE |
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