Controlled Release of Chemotherapeutic Platinum-Bisphosphonate Complexes from Injectable Calcium Phosphate Cements
| Autor: | Nicola Margiotta, Astghik Hayrapetyan, Jeroen J.J.P. van den Beucken, Michele Iafisco, Kemal Sariibrahimoglu, Kambiz Farbod, Sander C.G. Leeuwenburgh, Alessandra Curci, Jan N W Hakvoort |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Chemical structure
Kinetics Biomedical Engineering chemistry.chemical_element Bone Marrow Cells Bioengineering 02 engineering and technology Calcium 010402 general chemistry bone tumor 01 natural sciences Biochemistry hydroxyapatite nanoparticle Biomaterials chemistry.chemical_compound platinum-bisphosphonate Cell Line Tumor Humans injectable calcium phosphate cement Platinum Diphosphonates Bone Cements technology industry and agriculture Mesenchymal Stem Cells 021001 nanoscience & nanotechnology Controlled release In vitro 0104 chemical sciences PLGA Durapatite Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10] chemistry Delayed-Action Preparations Cancer cell Nanoparticles 0210 nano-technology controlled release Biomedical engineering Nuclear chemistry |
| Zdroj: | Tissue engineering. Part A 22 (2016): 788–800. doi:10.1089/ten.tea.2016.0001 info:cnr-pdr/source/autori:Farbod, Kambiz; Sariibrahimoglu, Kemal; Curci, Alessandra; Hayrapetyan, Astghik; Hakvoort, Jan N. W.; van den Beucken, Jeroen J. J. P.; Iafisco, Michele; Margiotta, Nicola; Leeuwenburgh, Sander C. G./titolo:Controlled Release of Chemotherapeutic Platinum-Bisphosphonate Complexes from Injectable Calcium Phosphate Cements/doi:10.1089%2Ften.tea.2016.0001/rivista:Tissue engineering. Part A/anno:2016/pagina_da:788/pagina_a:800/intervallo_pagine:788–800/volume:22 Tissue Engineering Part A, 22, 788-800 Tissue Engineering Part A, 22, 9-10, pp. 788-800 |
| ISSN: | 1937-3341 |
| DOI: | 10.1089/ten.tea.2016.0001 |
| Popis: | Item does not contain fulltext Herein, we present a method to release chemotherapeutic platinum-bisphosphonate (Pt-BP) complexes from apatitic calcium phosphate cements (CPCs). Pt-BP-loaded hydroxyapatite nanoparticles (HA NPs) were added at different ratios to the powder phase of the cements, which contained poly(D,L-lactic-co-glycolic acid) (PLGA) microspheres as porogens to accelerate their degradation. In vitro release kinetics of Pt-BP complexes revealed that the release rate of Pt species can be tuned by varying the amount of drug-loaded HA NPs as well as modifying the chemical structure of the Pt-BP complex to tailor its affinity with HA NPs. In addition, the incorporation of PLGA microspheres into the CPCs increased the degradation rate of the materials without affecting the release rate of Pt species. Finally, the antiproliferative activity of the free Pt-BP complexes and Pt-BP-loaded CPCs was evaluated using both human osteosarcoma cancer cells (MG-63) and human bone marrow-derived mesenchymal stromal cells (h-BMMSCs). This study demonstrated that both free Pt-BP complexes and the releasates from the CPCs were antiproliferative in a dose-dependent manner. Moreover, their antiproliferative activity was higher on MG-63 cells compared to h-BMMSC primary cells. In summary, it was shown that injectable CPCs can be rendered chemotherapeutically active by incorporation of HA NPs loaded with HA-binding Pt-BP complexes. |
| Databáze: | OpenAIRE |
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